DEPÈGE-FARGEIX, Nathalie; JAVELLE, Marie; CHAMBRIER, Pierre; FRANGNE, Nathalie; GERENTES, Denise; PEREZ, Pascual; ROGOWSKY, Peter; VERNOUD, Vanessa

doi:10.1093/jxb/erq267

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DEPÈGE-FARGEIX, Nathalie
Reproduction et développement des plantes [RDP]

JAVELLE, Marie
Reproduction et développement des plantes [RDP]

CHAMBRIER, Pierre
Reproduction et développement des plantes [RDP]

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Ce document a été publié dans

Journal of Experimental Botany. 2011, vol. 62, n° 1, p. 293-305

Oxford University Press (OUP)

Résumé en anglais

OCL1 (OUTER CELL LAYER1) encodes a maize HD-ZIP class IV transcription factor (TF) characterized by the presence of a homeo DNA-binding domain (HD), a dimerization leucine zipper domain (ZIP), and a steroidogenic acute regulatory protein (StAR)-related lipid transfer domain (START) involved in lipid transport in animals but the function of which is still unknown in plants. By combining yeast and plant trans-activation assays, the transcriptional activation domain of OCL1 was localized to 85 amino acids in the N-terminal part of the START domain. Full-length OCL1 devoid of this activation domain is unable to trans-activate a reporter gene under the control of a minimal promoter fused to six repeats of the L1 box, a cis-element present in target genes of HD-ZIP IV TFs in Arabidopsis. In addition, ectopic expression of OCL1 leads to pleiotropic phenotypic aberrations in transgenic maize plants, the most conspicuous one being a strong delay in flowering time which is correlated with the misexpression of molecular markers for floral transition such as ZMM4 (Zea Mays MADS-box4) or DLF1 (DELAYED FLOWERING1). As suggested by the interaction in planta between OCL1 and SWI3C1, a bona fide subunit of the SWI/SNF complex, OCL1 may modulate transcriptional activity of its target genes by interaction with a chromatin remodelling complex.< Réduire

Mots clés

TRANSCRIPTION FACTOR

BIOLOGIE DU DEVELOPPEMENT

BIOLOGIE MOLECULAIRE

BIOLOGIE VEGETALE

DEVELOPPEMENT DES PLANTES

Mots clés en anglais

ACTIVATION DOMAIN

CHROMATIN REMODELLING

FLOWERING TIME

START