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Functional characterization of the HD-ZIP IV transcription factor OCL1 from maize

hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorDEPÈGE-FARGEIX, Nathalie
hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorJAVELLE, Marie
hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorCHAMBRIER, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFRANGNE, Nathalie
hal.structure.identifierBIOGEMMA
dc.contributor.authorGERENTES, Denise
hal.structure.identifierBIOGEMMA
dc.contributor.authorPEREZ, Pascual
hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorROGOWSKY, Peter
hal.structure.identifierReproduction et développement des plantes [RDP]
dc.contributor.authorVERNOUD, Vanessa
dc.date.issued2011
dc.identifier.issn0022-0957
dc.description.abstractEnOCL1 (OUTER CELL LAYER1) encodes a maize HD-ZIP class IV transcription factor (TF) characterized by the presence of a homeo DNA-binding domain (HD), a dimerization leucine zipper domain (ZIP), and a steroidogenic acute regulatory protein (StAR)-related lipid transfer domain (START) involved in lipid transport in animals but the function of which is still unknown in plants. By combining yeast and plant trans-activation assays, the transcriptional activation domain of OCL1 was localized to 85 amino acids in the N-terminal part of the START domain. Full-length OCL1 devoid of this activation domain is unable to trans-activate a reporter gene under the control of a minimal promoter fused to six repeats of the L1 box, a cis-element present in target genes of HD-ZIP IV TFs in Arabidopsis. In addition, ectopic expression of OCL1 leads to pleiotropic phenotypic aberrations in transgenic maize plants, the most conspicuous one being a strong delay in flowering time which is correlated with the misexpression of molecular markers for floral transition such as ZMM4 (Zea Mays MADS-box4) or DLF1 (DELAYED FLOWERING1). As suggested by the interaction in planta between OCL1 and SWI3C1, a bona fide subunit of the SWI/SNF complex, OCL1 may modulate transcriptional activity of its target genes by interaction with a chromatin remodelling complex.
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subjectTRANSCRIPTION FACTOR
dc.subjectBIOLOGIE DU DEVELOPPEMENT
dc.subjectBIOLOGIE MOLECULAIRE
dc.subjectBIOLOGIE VEGETALE
dc.subjectDEVELOPPEMENT DES PLANTES
dc.subject.enACTIVATION DOMAIN
dc.subject.enCHROMATIN REMODELLING
dc.subject.enFLOWERING TIME
dc.subject.enSTART
dc.title.enFunctional characterization of the HD-ZIP IV transcription factor OCL1 from maize
dc.typeArticle de revue
dc.identifier.doi10.1093/jxb/erq267
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Botanique
bordeaux.journalJournal of Experimental Botany
bordeaux.page293-305
bordeaux.volume62
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-02645440
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02645440v1
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