DORDET FRISONI, Emilie; MARENDA, Marc Serge; NOUVEL, Laurent Xavier; GUÉRILLOT, Romain; GLASER, Philippe; BLANCHARD, Alain; TARDY, Florence; SIRAND-PUGNET, Pascal; BARANOWSKI, Eric; CITTI, Christine

doi:10.1111/mmi.12341

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

DORDET FRISONI, Emilie
Interactions hôtes-agents pathogènes [Toulouse] [IHAP]

MARENDA, Marc Serge
University of Melbourne
Melbourne Veterinary School [Parkville, VIC, Australie] [MVS]

NOUVEL, Laurent Xavier
Interactions hôtes-agents pathogènes [Toulouse] [IHAP]

Langue

Article de revue

Ce document a été publié dans

Molecular Microbiology. 2013-09, vol. 89, n° 6, p. 1226-1239

Wiley

Résumé en anglais

Horizontal gene transfer (HGT) is a major force of microbial evolution but was long thought to be marginal in mycoplasmas. In silico detection of exchanged regions and of loci encoding putative Integrative Conjugative Elements (ICE) in several mycoplasma genomes challenged this view, raising the prospect of these simple bacteria being able to conjugate. Using the model pathogen Mycoplasma agalactiae, we demonstrated for the first time that one of these elements, ICEA, is indeed self-transmissible. As a hallmark of conjugative processes, ICEA transfers were DNase resistant and required viable cells. ICEA acquisition conferred ICE-negative strains with the new ability to conjugate, allowing the spread of ICEA. Analysis of transfer-deficient mutants indicated that this process requires an ICEA-encoded lipoprotein of unknown function, CDS14. Formation of a circular extrachromosomal intermediate and the subsequent chromosomal integration of ICEA involved CDS22, an ICEA-encoded product distantly related to the ISLre2 transposase family. Remarkably, ICEA has no specific or no preferential integration site, often resulting in gene disruptions. Occurrence of functional mycoplasma ICE offers these bacteria with a means for HGT, a phenomenon with far-reaching implications given their minute-size genome and the number of species that are pathogenic for a broad host-range.< Réduire

DOI

http://dx.doi.org/10.1111/mmi.12341

Project ANR

Echange de gènes entre pathogènes " minimaux " par transfert horizontal : mécanismes et impacts sur l'évolution, la virulence et l'adaptation à l'hôte - ANR-09-MIEN-0016

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332