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ICEA of Mycoplasma agalactiae: a new family of self-transmissible integrative elements that confers conjugative properties to the recipient strain

hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDORDET FRISONI, Emilie
hal.structure.identifierUniversity of Melbourne
hal.structure.identifierMelbourne Veterinary School [Parkville, VIC, Australie] [MVS]
dc.contributor.authorMARENDA, Marc Serge
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorNOUVEL, Laurent Xavier
hal.structure.identifierGénétique des génomes - Genetics of Genomes [UMR 3525]
dc.contributor.authorGUÉRILLOT, Romain
hal.structure.identifierGénétique des génomes - Genetics of Genomes [UMR 3525]
dc.contributor.authorGLASER, Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierMycoplasmoses des Ruminants [MYCO]
dc.contributor.authorTARDY, Florence
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorBARANOWSKI, Eric
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
dc.date.issued2013-09
dc.identifier.issn0950-382X
dc.description.abstractEnHorizontal gene transfer (HGT) is a major force of microbial evolution but was long thought to be marginal in mycoplasmas. In silico detection of exchanged regions and of loci encoding putative Integrative Conjugative Elements (ICE) in several mycoplasma genomes challenged this view, raising the prospect of these simple bacteria being able to conjugate. Using the model pathogen Mycoplasma agalactiae, we demonstrated for the first time that one of these elements, ICEA, is indeed self-transmissible. As a hallmark of conjugative processes, ICEA transfers were DNase resistant and required viable cells. ICEA acquisition conferred ICE-negative strains with the new ability to conjugate, allowing the spread of ICEA. Analysis of transfer-deficient mutants indicated that this process requires an ICEA-encoded lipoprotein of unknown function, CDS14. Formation of a circular extrachromosomal intermediate and the subsequent chromosomal integration of ICEA involved CDS22, an ICEA-encoded product distantly related to the ISLre2 transposase family. Remarkably, ICEA has no specific or no preferential integration site, often resulting in gene disruptions. Occurrence of functional mycoplasma ICE offers these bacteria with a means for HGT, a phenomenon with far-reaching implications given their minute-size genome and the number of species that are pathogenic for a broad host-range.
dc.description.sponsorshipEchange de gènes entre pathogènes " minimaux " par transfert horizontal : mécanismes et impacts sur l'évolution, la virulence et l'adaptation à l'hôte - ANR-09-MIEN-0016
dc.language.isoen
dc.publisherWiley
dc.title.enICEA of Mycoplasma agalactiae: a new family of self-transmissible integrative elements that confers conjugative properties to the recipient strain
dc.typeArticle de revue
dc.identifier.doi10.1111/mmi.12341
dc.subject.halSciences du Vivant [q-bio]/Autre [q-bio.OT]
bordeaux.journalMolecular Microbiology
bordeaux.page1226-1239
bordeaux.volume89
bordeaux.issue6
bordeaux.peerReviewedoui
hal.identifierhal-02648702
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02648702v1
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