BALDET, Pierre; BRES, Cécile; OKABE, Yoshihiro; MAUXION, Jean-Philippe; JUST, Daniel; BOURNONVILLE, Celine; FERRAND, Carine; MORI, Kentaro; EZURA, Hiroshi; ROTHAN, Christophe

doi:10.5511/plantbiotechnology.13.0622b

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BALDET, Pierre
Biologie du fruit et pathologie [BFP]

BRES, Cécile
Biologie du fruit et pathologie [BFP]

OKABE, Yoshihiro
Université de Tsukuba = University of Tsukuba

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Article de revue

Ce document a été publié dans

Plant Biotechnology. 2013, vol. 30, n° 3, p. 309-314

Japanese Society for Plant Cell and Molecular Biology

Résumé en anglais

Besides being a model for fleshy fruits and Solanaceae species, tomato represents one of the main sources of ascorbate (vitamin C) in human diet in many parts of the world. Ascorbate fulfills various roles in plants due to its antioxidant potential and to its connection with other metabolic pathways e. g. cell wall biosynthesis. Among the functional genomic tools recently developed in tomato, EMS (ethyl methanesulfonate) mutant collections provide an opportunity for identifying allelic series of mutations in target genes by TILLING (Targeting Induced Local Lesions IN Genomes). We describe here the use of tomato EMS mutant collections in the miniature cv. Micro-Tom for the discovery of allelic variants in three ascorbate biosynthetic genes encoding the GDP-D-mannose pyrophosphorylase (GMP), the GDP-D-mannose epimerase (GME) and the GDP-L-galactose phosphorylase (GGP) respectively. We report on the discovery of several missense, truncation and splice junction mutations in these genes affecting plant ascorbate content to various levels, and show that several tomato mutant lines with strongly reduced ascorbate content undergo severe bleaching upon exposure to high light intensity.< Réduire

Mots clés

vitamin C

mutant

fruit

expression

mutation

Mots clés en anglais

gene

acid

arabidopsis

higher plant

l galactose phosphorylase

gdp d mannose pyrophosphorylase gmp

gdp d mannose epimerase gme

tilling

tomato

GDP-L-galactose phosphorylase

antioxidant

ascorbate

biosynthesis

sensitivity