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Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorOKABE, Yoshihiro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJUST, Daniel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOURNONVILLE, Celine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorFERRAND, Carine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierUniversité de Tsukuba = University of Tsukuba
dc.contributor.authorEZURA, Hiroshi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2013
dc.identifier.issn1342-4580
dc.description.abstractEnBesides being a model for fleshy fruits and Solanaceae species, tomato represents one of the main sources of ascorbate (vitamin C) in human diet in many parts of the world. Ascorbate fulfills various roles in plants due to its antioxidant potential and to its connection with other metabolic pathways e. g. cell wall biosynthesis. Among the functional genomic tools recently developed in tomato, EMS (ethyl methanesulfonate) mutant collections provide an opportunity for identifying allelic series of mutations in target genes by TILLING (Targeting Induced Local Lesions IN Genomes). We describe here the use of tomato EMS mutant collections in the miniature cv. Micro-Tom for the discovery of allelic variants in three ascorbate biosynthetic genes encoding the GDP-D-mannose pyrophosphorylase (GMP), the GDP-D-mannose epimerase (GME) and the GDP-L-galactose phosphorylase (GGP) respectively. We report on the discovery of several missense, truncation and splice junction mutations in these genes affecting plant ascorbate content to various levels, and show that several tomato mutant lines with strongly reduced ascorbate content undergo severe bleaching upon exposure to high light intensity.
dc.language.isoen
dc.publisherJapanese Society for Plant Cell and Molecular Biology
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectvitamin C
dc.subjectmutant
dc.subjectfruit
dc.subjectexpression
dc.subjectmutation
dc.subject.engene
dc.subject.enacid
dc.subject.enarabidopsis
dc.subject.enhigher plant
dc.subject.enl galactose phosphorylase
dc.subject.engdp d mannose pyrophosphorylase gmp
dc.subject.engdp d mannose epimerase gme
dc.subject.entilling
dc.subject.entomato
dc.subject.enGDP-L-galactose phosphorylase
dc.subject.enantioxidant
dc.subject.enascorbate
dc.subject.enbiosynthesis
dc.subject.ensensitivity
dc.title.enInvestigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants
dc.typeArticle de revue
dc.identifier.doi10.5511/plantbiotechnology.13.0622b
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Biotechnology
bordeaux.page309-314
bordeaux.volume30
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-02650242
hal.version2
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02650242v2
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Biotechnology&rft.date=2013&rft.volume=30&rft.issue=3&rft.spage=309-314&rft.epage=309-314&rft.eissn=1342-4580&rft.issn=1342-4580&rft.au=BALDET,%20Pierre&BRES,%20C%C3%A9cile&OKABE,%20Yoshihiro&MAUXION,%20Jean-Philippe&JUST,%20Daniel&rft.genre=article


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