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Redox-Immunofunctionalized Potyvirus Nanoparticles for High-Resolution Imaging by AFM-SECM Correlative Microscopy.

hal.structure.identifierUniversité Paris Diderot - Paris 7 [UPD7]
dc.contributor.authorANNE, Agnès
hal.structure.identifierUniversité Paris Diderot - Paris 7 [UPD7]
dc.contributor.authorCHOVIN, Arnaud
hal.structure.identifierUniversité Paris Diderot - Paris 7 [UPD7]
dc.contributor.authorDEMAILLE, Christophe
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMICHON, Thierry
dc.date.issued2018
dc.identifier.isbn978-1-4939-7806-9
dc.description.abstractEnWe present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence of ferrocene functionalized specific antibodies on the surface of potyvirus particles. For this purpose, potyviruses, flexuous filamentous phytoviruses with a high aspect ratio, have been chosen. This technique allows analysis of the distribution of antibody labeling over the virus population. But, more importantly, it opens up the imaging of immune complexes decorating a single viral particle. Finally, its high resolution allows the characterization in situ of the ultrastructure of a single immune complex on the particle.
dc.description.sponsorshipImagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009
dc.language.isoen
dc.publisherHumana Press
dc.publisher.locationNew York (united states)
dc.source.titleVirus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols
dc.subjectElectrochemical atomic force microscopy
dc.subjectLettuce mosaic virus (LMV)
dc.subject.enPotyvirus
dc.subject.enRedox antibodies
dc.subject.enSingle viral protein imaging
dc.subject.enViral nanoparticles
dc.title.enRedox-Immunofunctionalized Potyvirus Nanoparticles for High-Resolution Imaging by AFM-SECM Correlative Microscopy.
dc.typeChapitre d'ouvrage
dc.identifier.doi10.1007/978-1-4939-7808-3_30
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
dc.subject.halSciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique
bordeaux.volume1776
bordeaux.title.proceedingVirus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols
hal.identifierhal-02791110
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02791110v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Virus-Derived%20Nanoparticles%20for%20Advanced%20Technologies%20:%20Methods%20and%20Protocols&rft.date=2018&rft.volume=1776&rft.au=ANNE,%20Agn%C3%A8s&CHOVIN,%20Arnaud&DEMAILLE,%20Christophe&MICHON,%20Thierry&rft.isbn=978-1-4939-7806-9&rft.genre=unknown


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