PIANELLI, Katia; MONIER, Antoine; ANDRIEU, Marie-Hélène; BEAUVOIT, Bertrand; DIEUAIDE NOUBHANI, Martine

doi:10.1007/978-1-62703-688-7_3

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

PIANELLI, Katia
Biologie du fruit et pathologie [BFP]

MONIER, Antoine
Biologie du fruit et pathologie [BFP]

ANDRIEU, Marie-Hélène
Biologie du fruit et pathologie [BFP]

Langue

Chapitre d'ouvrage

Ce document a été publié dans

Plant Metabolic Flux Analysis : Methods and Protocols, Plant Metabolic Flux Analysis : Methods and Protocols. 2014, vol. 1090

Springer Science+Business Media

Résumé en anglais

Steady state (13)C-MFA is classically used to measure fluxes in complex metabolic networks. However, the modeling of steady state labeling allows the quantification of internal fluxes only and requires the estimation, by other methods, of the external fluxes, corresponding to substrate uptake (carbon input into the network) and to the production rate of compounds that accumulate within plant cells (network output). Additionally, it is not always possible to discriminate between different pathways that lead to the same label distribution. Methods to measure fluxes, based on direct measurements of pool size and on (14)C short-time labeling experiments, are described in this chapter. To illustrate this approach, we focus on the quantification of sucrose and starch turnovers.< Réduire

Mots clés en anglais

14C labeling

substrate cycle

sucrose turnover

Métadonnées

Licence d’utilisation du document

(14)C pulse labeling to estimate external fluxes and turnovers in primary metabolism.

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

DOI

Origine

Unités de recherche