(14)C pulse labeling to estimate external fluxes and turnovers in primary metabolism.
Language
en
Chapitre d'ouvrage
This item was published in
Plant Metabolic Flux Analysis : Methods and Protocols, Plant Metabolic Flux Analysis : Methods and Protocols. 2014, vol. 1090
Springer Science+Business Media
English Abstract
Steady state (13)C-MFA is classically used to measure fluxes in complex metabolic networks. However, the modeling of steady state labeling allows the quantification of internal fluxes only and requires the estimation, by ...Read more >
Steady state (13)C-MFA is classically used to measure fluxes in complex metabolic networks. However, the modeling of steady state labeling allows the quantification of internal fluxes only and requires the estimation, by other methods, of the external fluxes, corresponding to substrate uptake (carbon input into the network) and to the production rate of compounds that accumulate within plant cells (network output). Additionally, it is not always possible to discriminate between different pathways that lead to the same label distribution. Methods to measure fluxes, based on direct measurements of pool size and on (14)C short-time labeling experiments, are described in this chapter. To illustrate this approach, we focus on the quantification of sucrose and starch turnovers.Read less <
English Keywords
14C labeling
substrate cycle
sucrose turnover
Origin
Hal importedCollections