Mapping the phytoplasma chromosome
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | MALEMBIC-MAHER, Sylvie | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | CARLE, Patricia | |
| dc.date.issued | 2013 | |
| dc.identifier.isbn | 978-1-62703-088-5 978-1-62703-089-2 | |
| dc.description.abstractEn | Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example. | |
| dc.language.iso | en | |
| dc.publisher | Springer Science+Business Media | |
| dc.publisher.location | New York (united states) | |
| dc.source.title | Phytoplasma: Methods and Protocols | |
| dc.subject | flavescence dorée | |
| dc.subject | mollicutes | |
| dc.subject.en | Chromosome Mapping/ methods | |
| dc.subject.en | phytoplasma | |
| dc.subject.en | genetic map | |
| dc.subject.en | physical map | |
| dc.subject.en | pulsed-field gel electrophoresis | |
| dc.subject.en | restriction endonucleases | |
| dc.subject.en | southern blot | |
| dc.title.en | Mapping the phytoplasma chromosome | |
| dc.type | Chapitre d'ouvrage | |
| dc.identifier.doi | 10.1007/978-1-62703-089-2_34 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
| bordeaux.volume | 938 | |
| bordeaux.title.proceeding | Phytoplasma: Methods and Protocols | |
| hal.identifier | hal-02804649 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02804649v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Phytoplasma:%20Methods%20and%20Protocols&rft.date=2013&rft.volume=938&rft.au=MALEMBIC-MAHER,%20Sylvie&CARLE,%20Patricia&rft.isbn=978-1-62703-088-5%20978-1-62703-089-2&rft.genre=unknown |
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