Plant metabolomics and its potential for systems biology research: background concepts, technology, and methodology
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Methods in Systems Biology, Methods in Systems Biology. 2011, vol. 500
Elsevier Ltd
Resumen en inglés
The “metabolome” comprises the entire complement of small molecules in a plant or any other organism. It represents the ultimate phenotype of cells, deduced from the perturbation of gene expression and the modulation of ...Leer más >
The “metabolome” comprises the entire complement of small molecules in a plant or any other organism. It represents the ultimate phenotype of cells, deduced from the perturbation of gene expression and the modulation of protein function, as well as environmental cues. Extensive advances over the past decade, regarding the high-throughput (HTP) nature of “omics” research, have given birth to the expectation that a type of “systems level” overview may soon be possible. Having such a global overview of the molecular organization of a plant in the context of a particular set of genetic or environmental conditions, be it at cell, organ, or whole plant level, would clearly be very powerful. Currently, we are far from achieving this goal; however, within our hands, plant metabolomics is an HTP and informative “omics” approach to both sample generation and data generation, as well as raw data preprocessing, statistical analysis, and biological interpretation. Within this chapter, we aim to describe the great attention given to experimental design to ensure that the correct sample set and control are included and to, thereby, enable reliable statistical analysis of the data. For as comprehensive metabolite coverage as possible, we advocate the use of multiparallel approaches; thus, we describe a step-by-step standardized method for Nuclear magnetic resonance spectroscopy, as well as discussing with reference to standardized methodologies the techniques of gas chromatography-time of flight/mass spectrometry, and liquid chromatography–mass spectrometry.< Leer menos
Palabras clave
BIOLOGIE VEGETALE
RMN
GENERATION DE DONNEES
Palabras clave en inglés
GC-MS
LC-MS
GENERATION D'ECHANTILLONS
Orígen
Importado de HalCentros de investigación