Surface-immobilized fd bacteriophage particles are used as scaffolds to coassemble the redox enzyme quinoprotein glucose dehydrogenase, PQQ-GDH, and its cosubstrate, PEG-tethered ferrocene. Individual decorated fd phages are visualized and simultaneously functionally interrogated by Mt/AFM-SECM microscopy, an in-situ local probe correlative imaging technique, combining atomic force (AFM) and electrochemical (SECM) microscopy in a mediator tethered (Mt) configuration. The statistical distribution of catalytic activity across the fd population is resolved, and the correlation between the functional properties of the phages and their actual dimensions assessed. Moreover, achievement of sub-particle resolution allows the enzymatic activity of individual viruses to be spatially mapped, revealing a highly active region located in the middle of the filamentous fd-scaffold. Quantitative modeling shows that this “catalytic hot-spot” arises from the interplay between charge transport by electron hopping between ferrocene moieties along the viral particles, and enzymatic catalysis. The developed model also enables complete analysis of GDH kinetics at the single bioscaffold scale, revealing differences in the functional behavior of the biocatalytic viral particles when addressed at the ensemble or at the single particle scale.< Leer menos