UMBER, Marie; FILLOUX, Denis; GÉLABALE, Suzia; GOMEZ, Rose-Marie; MARAIS, Armelle; GALLET, Séverine; GAMIETTE, Franciane; PAVIS, Claudie; TEYCHENEY, Pierre-Yves

doi:10.3390/v12101101

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UMBER, Marie
Agrosystèmes tropicaux [ASTRO]

FILLOUX, Denis
Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
Département Systèmes Biologiques [Cirad-BIOS]

GÉLABALE, Suzia
Agrosystèmes tropicaux [ASTRO]

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Article de revue

Ce document a été publié dans

Viruses. 2020, vol. 12, n° 10, p. 1101

MDPI

Résumé en anglais

Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.< Réduire

Mots clés

virologie végétale

virus phytopathogène

santé des plantes

pathologie végétale

Mots clés en anglais

molecular diagnosis

yam

viruses

sanitation

germplasm conservation and dissemination