Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange
FILLOUX, Denis
Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
Département Systèmes Biologiques [Cirad-BIOS]
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Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
Département Systèmes Biologiques [Cirad-BIOS]
FILLOUX, Denis
Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
Département Systèmes Biologiques [Cirad-BIOS]
Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
Département Systèmes Biologiques [Cirad-BIOS]
TEYCHENEY, Pierre-Yves
Amélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
Département Systèmes Biologiques [Cirad-BIOS]
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Amélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
Département Systèmes Biologiques [Cirad-BIOS]
Language
en
Article de revue
This item was published in
Viruses. 2020, vol. 12, n° 10, p. 1101
MDPI
English Abstract
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report ...Read more >
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.Read less <
Keywords
virologie végétale
virus phytopathogène
santé des plantes
pathologie végétale
English Keywords
molecular diagnosis
yam
viruses
sanitation
germplasm conservation and dissemination
Origin
Hal importedCollections