RAVELONANDRO, Michel; BRIARD, Pascal; SCORZA, Ralph; CALLAHAN, Ann; ZAGRAI, Ioan; KUNDU, Jiban; DARDICK, Chris

doi:10.3390/genes12060816

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RAVELONANDRO, Michel
Biologie du fruit et pathologie [BFP]

BRIARD, Pascal
Biologie du fruit et pathologie [BFP]

SCORZA, Ralph
USDA-ARS : Agricultural Research Service

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Ce document a été publié dans

Genes. 2021-05-27, vol. 12, n° 6, p. 816

MDPI

Résumé en anglais

Our goal was to target silencing of the Plum pox virus coat protein (PPV CP) gene independently expressed in plants. Clone C-2 is a transgenic plum expressing CP. We introduced and verified, in planta, the effects of the inverse repeat of CP sequence split by a hairpin (IRSH) that was characterized in the HoneySweet plum. The IRSH construct was driven by two CaMV35S promoter sequences flanking the CP sequence and had been introduced into C1738 plum. To determine if this structure was enough to induce silencing, cross-hybridization was made with the C1738 clone and the CP expressing but PPV-susceptible C2 clone. In total, 4 out of 63 clones were silenced. While introduction of the IRSH is reduced due to the heterozygous character in C1738 plum, the silencing induced by the IRSH PPV CP is robust. Extensive studies, in greenhouse containment, demonstrated that the genetic resource of C1738 clone can silence the CP production. In addition, these were verified through the virus transgene pyramiding in the BO70146 BlueByrd cv. plum that successfully produced resistant BlueByrd BO70146 × C1738 (HybC1738) hybrid plums.< Réduire

Mots clés

virus phytopathogène

virologie végétale

pathologie végétale

arbre fruitier à noyau

fruit

Mots clés en anglais

Plum pox virus

Prunus domestica

resistance

RNAi

gene construct

hairpin