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Robust Response to Plum pox virus Infection via Plant Biotechnology

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRAVELONANDRO, Michel
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRIARD, Pascal
hal.structure.identifierUSDA-ARS : Agricultural Research Service
dc.contributor.authorSCORZA, Ralph
hal.structure.identifierUSDA-ARS : Agricultural Research Service
dc.contributor.authorCALLAHAN, Ann
hal.structure.identifierFruit Research and Development Station Bistrita
dc.contributor.authorZAGRAI, Ioan
hal.structure.identifierCrop research institute
dc.contributor.authorKUNDU, Jiban
hal.structure.identifierUSDA-ARS : Agricultural Research Service
dc.contributor.authorDARDICK, Chris
dc.date.accessioned2022-10-14T11:36:22Z
dc.date.available2022-10-14T11:36:22Z
dc.date.issued2021-05-27
dc.identifier.issn2073-4425
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/168386
dc.description.abstractEnOur goal was to target silencing of the Plum pox virus coat protein (PPV CP) gene independently expressed in plants. Clone C-2 is a transgenic plum expressing CP. We introduced and verified, in planta, the effects of the inverse repeat of CP sequence split by a hairpin (IRSH) that was characterized in the HoneySweet plum. The IRSH construct was driven by two CaMV35S promoter sequences flanking the CP sequence and had been introduced into C1738 plum. To determine if this structure was enough to induce silencing, cross-hybridization was made with the C1738 clone and the CP expressing but PPV-susceptible C2 clone. In total, 4 out of 63 clones were silenced. While introduction of the IRSH is reduced due to the heterozygous character in C1738 plum, the silencing induced by the IRSH PPV CP is robust. Extensive studies, in greenhouse containment, demonstrated that the genetic resource of C1738 clone can silence the CP production. In addition, these were verified through the virus transgene pyramiding in the BO70146 BlueByrd cv. plum that successfully produced resistant BlueByrd BO70146 × C1738 (HybC1738) hybrid plums.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectvirus phytopathogène
dc.subjectvirologie végétale
dc.subjectpathologie végétale
dc.subjectarbre fruitier à noyau
dc.subjectfruit
dc.subject.enPlum pox virus
dc.subject.enPrunus domestica
dc.subject.enresistance
dc.subject.enRNAi
dc.subject.engene construct
dc.subject.enhairpin
dc.title.enRobust Response to Plum pox virus Infection via Plant Biotechnology
dc.typeArticle de revue
dc.identifier.doi10.3390/genes12060816
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Amélioration des plantes
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalGenes
bordeaux.page816
bordeaux.volume12
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.issue6
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-03282642
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03282642v1
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