GARCHERY, Cécile; GEST, Noé; DO, Phuc; ALHAGDOW, Moftah; BALDET, Pierre; MENARD, Guillaume; ROTHAN, Christophe; MASSOT, Capucine; GAUTIER, Hélène; AARROUF, Jawad; FERNIE, Alisdair; STEVENS, Rebecca

doi:10.1111/j.1365-3040.2012.02564.x

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GARCHERY, Cécile
Génétique et Amélioration des Fruits et Légumes [GAFL]

GEST, Noé
Génétique et Amélioration des Fruits et Légumes [GAFL]

DO, Phuc
Max Planck Institute of Molecular Plant Physiology [MPI-MP]

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Plant, Cell and Environment. 2012-07-18, vol. 36, n° 1, p. 159 - 175

Wiley

Résumé en anglais

The regulation of carbon allocation between photosynthetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA interference was used to decrease ascorbate oxidase activity in tomato (Solanum lycopersicum L.). Fruit yield was increased in these lines under three conditions where assimilate became limiting for wild-type plants: when fruit trusses were left unpruned, when leaves were removed or when water supply was limited. Several alterations in the transgenic lines could contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. Ascorbate oxidase plants showed increases in stomatal conductance and leaf and fruit sugar content, as well as an altered apoplastic hexose : sucrose ratio. Modifications in gene expression, enzyme activity and the fruit metabolome were coherent with the notion of the ascorbate oxidase RNAi lines showing altered sink strength. Ascorbate oxidase may therefore be a target for strategies aimed at improving water productivity in crop species.< Réduire

Mots clés en anglais

Solanum lycopersicum

apoplast

drought

growth

oxidative stress

redox

RNAi

source-sink

water

diversity

adaptation

vitamin C

VTC: Vitamin C

AsA: Ascorbic acid

GME: GDP-d-mannose-3

5 -epimerase

GalLDH: L-Galactono-1

4-Lactone Dehydrogenase

AO: Ascorbate oxidase

MDHAR

Monodehydroascorbate reductase Ascorbic acid

Tomato

Cell wall

Complex systems

Systems biology Ascorbate

Tomate

Paroi

Systèmes complexes

Biologie intégrative

Ascorbate

cell wall

GDP-D-mannose epimerase

rhamnogalacturonan-II

Solanum lycopersicum

tomato fruits AsA: ascorbic acid

DPA: days post anthesis

PMI: phosphomannose isomerase

GMP: GDP-D-mannose pyrophosphorylase

GME: GDP-D-mannose-3

5-epimerase

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A diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit

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Résumé en anglais

Mots clés en anglais

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