Genome Engineering in Mycoplasma gallisepticum Using Exogenous Recombination Systems
Langue
en
Article de revue
Ce document a été publié dans
ACS Synthetic Biology. 2022, vol. 11, p. 1060-1067
American Chemical Society
Résumé en anglais
Mycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of Mgal virulence and host ...Lire la suite >
Mycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of Mgal virulence and host specificity are still largely unknown, mostly due to the absence of efficient methods for functional genomics. After evaluating two exogenous recombination systems derived from phages found in the phylogenetically related Spiroplasma phoeniceum and the more distant Bacillus subtilis, the RecET-like system from B. subtilis was successfully used for gene inactivation and targeted replacement in Mgal. In a second step, the Cre-lox recombination system was used for the removal of the antibiotic resistance marker in recombinant mutants. This study therefore describes the first genetic tool for targeted genome engineering of Mgal and demonstrates the efficiency of heterologous recombination systems in minimal bacteria.< Réduire
Mots clés en anglais
Mollicutes
Mycoplasma gallisepticum
RecET
mutagenesis
poultry
recombination
Origine
Importé de halUnités de recherche