Genome Engineering in Mycoplasma gallisepticum Using Exogenous Recombination Systems
Language
en
Article de revue
This item was published in
ACS Synthetic Biology. 2022, vol. 11, p. 1060-1067
American Chemical Society
English Abstract
Mycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of Mgal virulence and host ...Read more >
Mycoplasma gallisepticum (Mgal) is a common pathogen of poultry worldwide that has recently spread to North American house finches after a single host shift in 1994. The molecular determinants of Mgal virulence and host specificity are still largely unknown, mostly due to the absence of efficient methods for functional genomics. After evaluating two exogenous recombination systems derived from phages found in the phylogenetically related Spiroplasma phoeniceum and the more distant Bacillus subtilis, the RecET-like system from B. subtilis was successfully used for gene inactivation and targeted replacement in Mgal. In a second step, the Cre-lox recombination system was used for the removal of the antibiotic resistance marker in recombinant mutants. This study therefore describes the first genetic tool for targeted genome engineering of Mgal and demonstrates the efficiency of heterologous recombination systems in minimal bacteria.Read less <
English Keywords
Mollicutes
Mycoplasma gallisepticum
RecET
mutagenesis
poultry
recombination
Origin
Hal importedCollections