GRAUBY-HEYWANG, Christine; MOROTÉ, Fabien; MATHELIÉ-GUINLET, Marion; GAMMOUDI, Ibtissem; FAYE, Ndeye Rokhaya; COHEN-BOUHACINA, Touria

doi:10.1016/j.chemphyslip.2016.07.001

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

http://creativecommons.org/licenses/by-sa/

GRAUBY-HEYWANG, Christine
Laboratoire Ondes et Matière d'Aquitaine [LOMA]

MOROTÉ, Fabien
Laboratoire Ondes et Matière d'Aquitaine [LOMA]

MATHELIÉ-GUINLET, Marion
Laboratoire Ondes et Matière d'Aquitaine [LOMA]

Langue

Article de revue

Ce document a été publié dans

Chemistry and Physics of Lipids. 2016-10, vol. 200, p. 74 - 82

Elsevier

Résumé en anglais

In this work, we studied the interaction of two oxidized lipids, PoxnoPC and PazePC, with POPC phospholipid. Mean molecular areas obtained from (π–A) isotherms of mixed PoxnoPC–POPC and PazePC–POPC monolayers revealed different behaviors of these two oxidized lipids: the presence of PoxnoPC in the monolayers induces their expansion, mean molecular areas being higher than those expected in the case of ideal mixtures. PazePC–POPC behave on the whole ideally. This difference can be explained by a different conformation of oxidized lipids. Moreover the carboxylic function of PazePC is protonated under our experimental conditions, as shown by (π–A) isotherms of PazePC at different pH values. Both oxidized lipids induce also an increase of the monolayer elasticity, PoxnoPC being slightly more efficient than PazePC. These monolayers were transferred from the air–water interface onto mica supports for a study by AFM. AFM images are on the whole homogenous, suggesting the presence of only one lipid phase in both cases. However, in the case of PazePC–POPC monolayers, AFM images show also the presence of areas thicker of 7 nm to 10 nm than the surrounding lipid phase, probably due to the local formation of multilayer systems induced by compression.< Réduire

Mots clés en anglais

Oxidized lipid

Langmuir monolayer

Langmuir-Blodgett film

Surface pressure measurements

Atomic force microscopy

Métadonnées

Licence d’utilisation du document

Influence of oxidized lipids on palmitoyl-oleoyl-phosphatidylcholine organization, contribution of Langmuir monolayers and Langmuir–Blodgett films

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

DOI

Origine

Unités de recherche