ARBONA, Jean-Michel; GOLDAR, Arach; HYRIEN, Olivier; ARNEODO, Alain; AUDIT, Benjamin

doi:10.7554/eLife.35192

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ARBONA, Jean-Michel
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]

GOLDAR, Arach
Régulation transcriptionnelle des génomes [GTR]
Laboratoire de Biologie Intégrative [LBI]

HYRIEN, Olivier
Institut de biologie de l'ENS Paris [IBENS]

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eLife. 2018-06-01, vol. 7, p. e35192

eLife Sciences Publication

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The time-dependent rate I(t) of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firing factors. Here, we show that a normal diffusion of replication fork components towards localized potential replication origins (p-oris) can more simply account for the I(t) universal bell shape, as a consequence of a competition between the origin firing time and the time needed to replicate DNA separating two neighboring p-oris. We predict the I(t) maximal value to be the product of the replication fork speed with the squared p-ori density. We show that this relation is robustly observed in simulations and in experimental data for several eukaryotes. Our work underlines that fork-component recycling and potential origins localization are sufficient spatial ingredients to explain the universality of DNA replication kinetics.< Réduire

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The eukaryotic bell-shaped temporal rate of DNA replication origin firing emanates from a balance between origin activation and passivation

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