BEN ABDALLAH, Zeineb; SGHAIER, Halim; GAMMOUDI, Ibtissem; MOROTE, F.; CASSAGNÈRE, Sébastien; ROMO, Lena; BEVEN, Laure; GRAUBY-HEYWANG, Christine; COHEN-BOUHACINA, Touria

doi:10.3390/chemosensors10040137

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BEN ABDALLAH, Zeineb
Laboratoire Ondes et Matière d'Aquitaine [LOMA]

SGHAIER, Halim
Université de Monastir - University of Monastir [UM]

GAMMOUDI, Ibtissem
Laboratoire Ondes et Matière d'Aquitaine [LOMA]

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Chemosensors. 2022-04, vol. 10, n° 4, p. 137

MDPI

Résumé en anglais

This work describes the complete elaboration of an immunosensor for the detection of the fungal B1 aflatoxin (AFB1). In a first step, a system made of three screen-printed electrodes (SPEs) was manufactured using gold, silver/silver chloride, and carbon pastes. Raman spectroscopy showed that the thermal treatment applied to the electrodes enabled a strong decrease in the amount of undesirable organic molecules for each paste. Atomic Force Microscopy was also used to reveal the morphology of the electrode surfaces. In a second step, an autonomous and cheap electronic system was designed for the control of the sensor and electrochemical measurements, showing current variations significantly higher than those observed with a commercial system. In a last step, the gold working electrode of this system was functionalized by a simple self-assembly method, optimized in a previous work, with a molecular architecture including an antibody recognizing specifically AFB1. The complete device was finally realized by combining the SPEs and the electronic platform. The resulting setup was able to detect AFB1 toxin in a buffer with an LOD of about 50 fg/mL. It was then applied to the detection of AFB1 in rice milk, a more realistic medium comparable with those met in an agrifood context. The electrochemical detection of AFB1 was possible in a range of concentration between 0.5 pg/mL and 2.5 pg/mL, with the sensor behaving linearly in this range.< Réduire

Mots clés en anglais

AFB1 toxin

screen printing

electrochemical immunosensor

electronics

Ben Abdallah

Sghaier

Gammoudi

Moroté

Cassagnère

Romo

Béven

Grauby-Heywang

Cohen-Bouhacina

T. Design

Elaboration

and Characterization of Chemosensors 2022

137 AFB1 toxin

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Design, Elaboration, and Characterization of an Immunosensor for the Detection of a Fungal Toxin in Foodstuff Analyses

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