Design, Elaboration, and Characterization of an Immunosensor for the Detection of a Fungal Toxin in Foodstuff Analyses
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | BEN ABDALLAH, Zeineb | |
| hal.structure.identifier | Université de Monastir - University of Monastir [UM] | |
| dc.contributor.author | SGHAIER, Halim | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | GAMMOUDI, Ibtissem | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | MOROTE, F. | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | CASSAGNÈRE, Sébastien | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | ROMO, Lena | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BEVEN, Laure | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | GRAUBY-HEYWANG, Christine | |
| hal.structure.identifier | Laboratoire Ondes et Matière d'Aquitaine [LOMA] | |
| dc.contributor.author | COHEN-BOUHACINA, Touria | |
| dc.date.issued | 2022-04 | |
| dc.identifier.issn | 2227-9040 | |
| dc.description.abstractEn | This work describes the complete elaboration of an immunosensor for the detection of the fungal B1 aflatoxin (AFB1). In a first step, a system made of three screen-printed electrodes (SPEs) was manufactured using gold, silver/silver chloride, and carbon pastes. Raman spectroscopy showed that the thermal treatment applied to the electrodes enabled a strong decrease in the amount of undesirable organic molecules for each paste. Atomic Force Microscopy was also used to reveal the morphology of the electrode surfaces. In a second step, an autonomous and cheap electronic system was designed for the control of the sensor and electrochemical measurements, showing current variations significantly higher than those observed with a commercial system. In a last step, the gold working electrode of this system was functionalized by a simple self-assembly method, optimized in a previous work, with a molecular architecture including an antibody recognizing specifically AFB1. The complete device was finally realized by combining the SPEs and the electronic platform. The resulting setup was able to detect AFB1 toxin in a buffer with an LOD of about 50 fg/mL. It was then applied to the detection of AFB1 in rice milk, a more realistic medium comparable with those met in an agrifood context. The electrochemical detection of AFB1 was possible in a range of concentration between 0.5 pg/mL and 2.5 pg/mL, with the sensor behaving linearly in this range. | |
| dc.language.iso | en | |
| dc.publisher | MDPI | |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | AFB1 toxin | |
| dc.subject.en | screen printing | |
| dc.subject.en | electrochemical immunosensor | |
| dc.subject.en | electronics | |
| dc.subject.en | Ben Abdallah | |
| dc.subject.en | Z. | |
| dc.subject.en | Sghaier | |
| dc.subject.en | H. | |
| dc.subject.en | Gammoudi | |
| dc.subject.en | I. | |
| dc.subject.en | Moroté | |
| dc.subject.en | F. | |
| dc.subject.en | Cassagnère | |
| dc.subject.en | S. | |
| dc.subject.en | Romo | |
| dc.subject.en | L. | |
| dc.subject.en | Béven | |
| dc.subject.en | Grauby-Heywang | |
| dc.subject.en | C. | |
| dc.subject.en | Cohen-Bouhacina | |
| dc.subject.en | T. Design | |
| dc.subject.en | Elaboration | |
| dc.subject.en | and Characterization of Chemosensors 2022 | |
| dc.subject.en | 10 | |
| dc.subject.en | 137 AFB1 toxin | |
| dc.title.en | Design, Elaboration, and Characterization of an Immunosensor for the Detection of a Fungal Toxin in Foodstuff Analyses | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.3390/chemosensors10040137 | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| bordeaux.journal | Chemosensors | |
| bordeaux.page | 137 | |
| bordeaux.volume | 10 | |
| bordeaux.issue | 4 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-03764767 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-03764767v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Chemosensors&rft.date=2022-04&rft.volume=10&rft.issue=4&rft.spage=137&rft.epage=137&rft.eissn=2227-9040&rft.issn=2227-9040&rft.au=BEN%20ABDALLAH,%20Zeineb&SGHAIER,%20Halim&GAMMOUDI,%20Ibtissem&MOROTE,%20F.&CASSAGN%C3%88RE,%20S%C3%A9bastien&rft.genre=article |
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