Robust single-molecule approach for counting autofluorescent proteins.
Idioma
en
Article de revue
Este ítem está publicado en
Journal of Biomedical Optics. 2008-06-23, vol. 13, n° 3, p. 031216
Society of Photo-optical Instrumentation Engineers
Resumen en inglés
Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted ...Leer más >
Palabras clave en inglés
aggregation
bioluminescence
fluorescence
molecular biophysics
optical saturable absorption
proteins