TORRES RUIZ, Jose Manuel; COCHARD, Hervé; CHOAT, Brendan; JANSEN, Steven; LOPEZ RODRIGUEZ, Rosa Ana; TOMÁŠKOVÁ, Ivana; PADILLA-DÍAZ, Carmen M.; BADEL, Eric; BURLETT, Régis; KING, Andrew; LENOIR, Nicolas; MARTIN-STPAUL, Nicolas; DELZON, Sylvain

doi:10.1111/nph.14589

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TORRES RUIZ, Jose Manuel
Biodiversité, Gènes & Communautés [BioGeCo]

COCHARD, Hervé
Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]

CHOAT, Brendan
Hawkesbury Institute for the Environment [Richmond] [HIE]

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Article de revue

Ce document a été publié dans

New Phytologist. 2017, vol. 215, n° 1, p. np

Wiley

Résumé en anglais

Xylem vulnerability to embolism represents an essential trait for the evaluation of the impact of hydraulics in plant function and ecology. The standard centrifuge technique is widely used for the construction of vulnerability curves, although its accuracy when applied to species with long vessels remains under debate. We developed a simple diagnostic test to determine whether the open-vessel artefact influences centrifuge estimates of embolism resistance. Xylem samples from three species with differing vessel lengths were exposed to less negative xylem pressures via centrifugation than the minimum pressure the sample had previously experienced. Additional calibration was obtained from non-invasive measurement of embolism on intact olive plants by X-ray microtomography. Results showed artefactual decreases in hydraulic conductance (k) for samples with open vessels when exposed to a less negative xylem pressure than the minimum pressure they had previously experienced. X-Ray microtomography indicated that most of the embolism formation in olive occurs at xylem pressures below -4.0 MPa, reaching 50% loss of hydraulic conductivity at -5.3 MPa. The artefactual reductions in k induced by centrifugation underestimate embolism resistance data of species with long vessels. A simple test is suggested to avoid this open vessel artefact and to ensure the reliability of this technique in future studies.< Réduire

Mots clés

xylème

artefact

activité hydraulique

biologie de la plante

centrifugation

microtomographie

courbe de vulnérabilité

embolie des vaisseaux

Mots clés en anglais

centrifuge technique

embolism

micro-CT

olive

xylem

artifact

centrifuging

DOI

http://dx.doi.org/10.1111/nph.14589

Projet Européen

Plasticity and adaptation of hydraulic traits to overcome climate change

Project ANR

Plateforme d'Innovation " Forêt-Bois-Fibre-Biomasse du Futur "

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202