BARTHOLOMÉ, Jérôme; MANDROU, Eric; MABIALA, André; JENKINS, Jerry; NABIHOUDINE, Ibouniyamine; KLOPP, Christophe; SCHMUTZ, Jeremy; PLOMION, Christophe; GION, Jean-Marc

doi:10.1111/nph.13150

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BARTHOLOMÉ, Jérôme
Biodiversité, Gènes & Communautés [BioGeCo]
Amélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]

MANDROU, Eric
Biodiversité, Gènes & Communautés [BioGeCo]

MABIALA, André
Centre de Recherche sur la Durabilité et la Productivité des Plantations Industrielles [CRDPI]

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New Phytologist. 2015, vol. 206, n° 4, p. 1283-1296

Wiley

Résumé en anglais

Genetic maps are key tools in genetic research as they constitute the framework for many applications, such as quantitative trait locus analysis, and support the assembly of genome sequences. The resequencing of the two parents of a cross between Eucalyptus urophylla and Eucalyptus grandis was used to design a single nucleotide polymorphism (SNP) array of 6000 markers evenly distributed along the E. grandis genome. The genotyping of 1025 offspring enabled the construction of two high-resolution genetic maps containing 1832 and 1773 markers with an average marker interval of 0.45 and 0.5 cM for E. grandis and E. urophylla, respectively. The comparison between genetic maps and the reference genome highlighted 85% of collinear regions. A total of 43 noncollinear regions and 13 nonsynthetic regions were detected and corrected in the new genome assembly. This improved version contains 4943 scaffolds totalling 691.3 Mb of which 88.6% were captured by the 11 chromosomes. The mapping data were also used to investigate the effect of population size and number of markers on linkage mapping accuracy. This study provides the most reliable linkage maps for Eucalyptus and version 2.0 of the E. grandis genome.< Réduire

Mots clés en anglais

Eucalyptus

genetic mapping

genome assembly

segregation distortion

single nucleotide polymorphism (SNP) array

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High-resolution genetic maps of Eucalyptus improve Eucalyptus grandis genome assembly

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