JORGE, Véronique; DUPLESSIS, Sébastien; LALANNE, Céline; BRESSON, Alois; DOWKIW, Arnaud; FAIVRE-RAMPANT, Patricia; FREY, Pascal; GUÉRIN, Vanina; KOHLER, Annegret; MARTIN, Francis; PAOLUCCI, Isabella; PLOMION, Christophe; RINALDI, Cécile; BASTIEN, Catherine

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JORGE, Véronique
Unité de recherche Amélioration, Génétique et Physiologie Forestières [AGPF]

DUPLESSIS, Sébastien
Interactions Arbres-Microorganismes [IAM]

LALANNE, Céline
Biodiversité, Gènes & Communautés [BioGeCo]

Langue

Communication dans un congrès

Ce document a été publié dans

Plant genomics and beyond, 2009-07-05, Evry. 2009

Résumé en anglais

Foliar rust is the most devastating disease affecting European poplar plantations. One major QTL (Rus), identified in a Populus deltoides x P. trichocorpa pedigree, is on the process of positional cloning. To precise the molecular basis of Rus, joint proteomic and transcriptomic approaches were used to characterize differential expression 2 and 4 days post inoculation (dpi) in two bulks (Rus vs rus) of 10 progenies. At 2 dpi, few transcripts showed an altered expression in the Rus bulk, while genes known to be targeted by resistance genes in plant pathosystems, were found up-regulated 4 dpi. The rus bulk did not present such gene up-regulation while several transcripts, including defense-related genes, were down-regulated as early as 2 dpi. Changes in the proteome were studied by 2-D PAGE analysis and MS/MS characterization of a selected subset of 50 protein spots at 2 and 4 dpi. Interestingly, the proteins overexpressed in the Rus genotypes correlated quite well with the transcriptomic data. All the results, including data coming from positional cloning, suggested that Rus activates the same genes than those involved in qualitative resistance, but this activation is delayed in the quantitative response.< Réduire

Mots clés

QUANTITATIVE RESISTANCE

TRANSCRIPTOMICS

PROTEOMICS

Mots clés en anglais

FOLIAR RUST

POPLAR RUST

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202