Measuring xylem hydraulic vulnerability for long-vessel species: an improved methodology with the flow centrifugation technique
COCHARD, Hervé
Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
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Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
Langue
en
Article de revue
Ce document a été publié dans
Annals of Forest Science. 2022-03-17, vol. 79, n° 1, p. 16 p.
Springer Nature (since 2011)/EDP Science (until 2010)
Résumé en anglais
Context: Understanding plant resilience and adaptation to drought is a major challenge in crop and forest sciences. Several methods have been developed to assess the vulnerability to xylem embolism. The in situ flow ...Lire la suite >
Context: Understanding plant resilience and adaptation to drought is a major challenge in crop and forest sciences. Several methods have been developed to assess the vulnerability to xylem embolism. The in situ flow centrifuge (or cavitron) is the fastest technique allowing to characterise this trait for plants having vessel lengths shorter than the rotor size. Aims: We present (i) a series of changes to the earlier cavitron design, aimed at improving the accuracy and speed of measurement through automated operations, and (ii) a new development through the design of a large diameter rotor expanding the range of species that can be measured. Methods: Both hardware and software modifications to the original design have been developed. In order to avoid artefacts caused by cut open vessels, a centrifuge with a large rotor (1 m) has been developed, and vulnerability curves obtained with this new device were compared with those obtained using reference methods. Results: The new setup expands the range of conductance measurable with a cavitron and enables it to accurately determine the absolute value of conductivity even for species having very low hydraulic conductivity. The large rotor cavitron shows good agreement with the reference techniques for conifers and diffuse-porous species but also for ring-porous species having long vessels. Conclusion: The setup described in this manuscript provides a faster, safer and more accurate method to construct vulnerability curves, compared to the original cavitron design, and extends the measurement capabilities to new species that are difficult to measure to date. Key message: Recent improvements to cavitron setup enable to measure xylem vulnerability curves for an expanded number of plant species, with longer vessels or lower hydraulic conductivity.< Réduire
Mots clés en anglais
Drought resistance
Plant hydraulic
Vulnerability curve
Cavitron
Method
Project ANR
Plateforme d'Innovation " Forêt-Bois-Fibre-Biomasse du Futur " - ANR-10-EQPX-0016
COntinental To coastal Ecosystems: evolution, adaptability and governance - ANR-10-LABX-0045
COntinental To coastal Ecosystems: evolution, adaptability and governance - ANR-10-LABX-0045
Origine
Importé de halUnités de recherche