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Measuring xylem hydraulic vulnerability for long-vessel species: an improved methodology with the flow centrifugation technique

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorBURLETT, Régis
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPARISE, Camille
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCAPDEVILLE, Gaëlle
hal.structure.identifierLaboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant [PIAF]
dc.contributor.authorCOCHARD, Hervé
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLAMARQUE, Laurent
hal.structure.identifierSynchrotron SOLEIL [SSOLEIL]
dc.contributor.authorKING, Andrew
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorDELZON, Sylvain
dc.date.issued2022-03-17
dc.identifier.issn1286-4560
dc.description.abstractEnContext: Understanding plant resilience and adaptation to drought is a major challenge in crop and forest sciences. Several methods have been developed to assess the vulnerability to xylem embolism. The in situ flow centrifuge (or cavitron) is the fastest technique allowing to characterise this trait for plants having vessel lengths shorter than the rotor size. Aims: We present (i) a series of changes to the earlier cavitron design, aimed at improving the accuracy and speed of measurement through automated operations, and (ii) a new development through the design of a large diameter rotor expanding the range of species that can be measured. Methods: Both hardware and software modifications to the original design have been developed. In order to avoid artefacts caused by cut open vessels, a centrifuge with a large rotor (1 m) has been developed, and vulnerability curves obtained with this new device were compared with those obtained using reference methods. Results: The new setup expands the range of conductance measurable with a cavitron and enables it to accurately determine the absolute value of conductivity even for species having very low hydraulic conductivity. The large rotor cavitron shows good agreement with the reference techniques for conifers and diffuse-porous species but also for ring-porous species having long vessels. Conclusion: The setup described in this manuscript provides a faster, safer and more accurate method to construct vulnerability curves, compared to the original cavitron design, and extends the measurement capabilities to new species that are difficult to measure to date. Key message: Recent improvements to cavitron setup enable to measure xylem vulnerability curves for an expanded number of plant species, with longer vessels or lower hydraulic conductivity.
dc.description.sponsorshipPlateforme d'Innovation " Forêt-Bois-Fibre-Biomasse du Futur " - ANR-10-EQPX-0016
dc.description.sponsorshipCOntinental To coastal Ecosystems: evolution, adaptability and governance - ANR-10-LABX-0045
dc.language.isoen
dc.publisherSpringer Nature (since 2011)/EDP Science (until 2010)
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enDrought resistance
dc.subject.enPlant hydraulic
dc.subject.enVulnerability curve
dc.subject.enCavitron
dc.subject.enMethod
dc.title.enMeasuring xylem hydraulic vulnerability for long-vessel species: an improved methodology with the flow centrifugation technique
dc.typeArticle de revue
dc.identifier.doi10.1186/s13595-022-01124-0
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalAnnals of Forest Science
bordeaux.page16 p.
bordeaux.volume79
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03672717
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03672717v1
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