LARIVIÈRE, Mélusine; LORENZATO, Cyril Samuel; ADUMEAU, Laurent; BONNET, Samuel; HEMADOU, Audrey; JACOBIN-VALAT, Marie-Josée; NOUBHANI, Abdelmajid; SANTARELLI, Xavier; MINDER, Laetitia; DI PRIMO, Carmelo; SANCHEZ, Stephane; MORNET, Stéphane; LAROCHE-TRAINEAU, Jeanny; CLOFENT-SANCHEZ, Gisèle

doi:10.1016/j.nano.2019.102082

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LARIVIÈRE, Mélusine
Centre de résonance magnétique des systèmes biologiques [CRMSB]

LORENZATO, Cyril Samuel
Centre de résonance magnétique des systèmes biologiques [CRMSB]

ADUMEAU, Laurent
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]

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Article de revue

Ce document a été publié dans

Nanomedicine: Nanotechnology, Biology and Medicine. 2019-11, vol. 22, p. 102082 (12 p.)

Elsevier

Résumé en anglais

Due to the wealth of actors involved in the development of atherosclerosis, molecular imaging based on the targeting of specific markers would substantiate the diagnosis of life-threatening atheroma plaques. To this end, TEG4 antibody is a promising candidate targeting the activated platelets (integrin αIIbβ3) highly represented within the plaque. In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE−/− mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their value as a potential diagnosis agent.< Réduire

Mots clés en anglais

Atherosclerosis

Bimodal imaging

scFv antibody fragments

Directed conjugation

SPIO

Near infrared fluorescence (NIRF)

DOI

http://dx.doi.org/10.1016/j.nano.2019.102082

Project ANR

Développement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004

Origine

Importé de hal

Unités de recherche

Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB) - UMR 5026