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Multimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody

hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorLARIVIÈRE, Mélusine
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorLORENZATO, Cyril Samuel
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorADUMEAU, Laurent
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBONNET, Samuel
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorHEMADOU, Audrey
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorJACOBIN-VALAT, Marie-Josée
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorNOUBHANI, Abdelmajid
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSANTARELLI, Xavier
hal.structure.identifierInstitut Européen de Chimie et de Biologie
hal.structure.identifierBordeaux - Recherche Intégrée en Oncologie [SIRIC-BRIO]
dc.contributor.authorMINDER, Laetitia
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorDI PRIMO, Carmelo
hal.structure.identifierAcides Nucléiques : Régulations Naturelle et Artificielle [ARNA]
dc.contributor.authorSANCHEZ, Stephane
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorMORNET, Stéphane
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorLAROCHE-TRAINEAU, Jeanny
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorCLOFENT-SANCHEZ, Gisèle
dc.date.issued2019-11
dc.identifier.issn1549-9634
dc.description.abstractEnDue to the wealth of actors involved in the development of atherosclerosis, molecular imaging based on the targeting of specific markers would substantiate the diagnosis of life-threatening atheroma plaques. To this end, TEG4 antibody is a promising candidate targeting the activated platelets (integrin αIIbβ3) highly represented within the plaque. In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE−/− mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their value as a potential diagnosis agent.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée coordonnée
dc.language.isoen
dc.publisherElsevier
dc.subject.enAtherosclerosis
dc.subject.enBimodal imaging
dc.subject.enscFv antibody fragments
dc.subject.enDirected conjugation
dc.subject.enSPIO
dc.subject.enNear infrared fluorescence (NIRF)
dc.title.enMultimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody
dc.typeArticle de revue
dc.identifier.doi10.1016/j.nano.2019.102082
dc.subject.halChimie/Matériaux
bordeaux.journalNanomedicine: Nanotechnology, Biology and Medicine
bordeaux.page102082 (12 p.)
bordeaux.volume22
bordeaux.peerReviewedoui
hal.identifierhal-02286566
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02286566v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nanomedicine:%20Nanotechnology,%20Biology%20and%20Medicine&rft.date=2019-11&rft.volume=22&rft.spage=102082%20(12%20p.)&rft.epage=102082%20(12%20p.)&rft.eissn=1549-9634&rft.issn=1549-9634&rft.au=LARIVI%C3%88RE,%20M%C3%A9lusine&LORENZATO,%20Cyril%20Samuel&ADUMEAU,%20Laurent&BONNET,%20Samuel&HEMADOU,%20Audrey&rft.genre=article


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