BONNET, Jacques; COLOTTE, Marthe; COUDY, Delphine; COUALLIER, Vincent; PORTIER, Josik; MORIN, Bénédicte; TUFFET, Sophie

doi:10.1093/nar/gkp1060

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BONNET, Jacques
Validation et identification de nouvelles cibles en oncologie [VINCO]

COLOTTE, Marthe
Plateforme génomique fonctionnelle [PGFB]
Société IMAGENE

COUDY, Delphine
Société IMAGENE

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Nucleic Acids Research. 2010, vol. 38, n° 5, p. 1531-1546

Oxford University Press

Résumé en anglais

There is currently wide interest in room temperature storage of dehydrated DNA. However, there is insufficient knowledge about its chemical and structural stability. Here, we show that solid-state DNA degradation is greatly affected by atmospheric water and oxygen at room temperature. In these conditions DNA can even be lost by aggregation. These are major concerns since laboratory plastic ware is not airtight. Chain-breaking rates measured between 70 C and 140 C seemed to follow Arrhenius' law. Extrapolation to 25 C gave a degradation rate of about 1-40 cuts/105 nucleotides/ century. However, these figures are to be taken as very tentative since they depend on the validity of the extrapolation and the positive or negative effect of contaminants, buffers or additives. Regarding the secondary structure, denaturation experiments showed that DNA secondary structure could be preserved or fully restored upon rehydration, except possibly for small fragments. Indeed, below about 500 bp, DNA fragments underwent a very slow evolution (almost suppressed in the presence of trehalose) which could end in an irreversible denaturation. Thus, this work validates using room temperature for storage of DNA if completely protected from water and oxygen.< Réduire

Mots clés en anglais

DNA degradation

Chain-breaking rates

Ancient DNA

Accelerated degradation

Arrhenius' law

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Chain and conformation stability of solid-state DNA: implications for room temperature storage

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