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Chain and conformation stability of solid-state DNA: implications for room temperature storage

hal.structure.identifierValidation et identification de nouvelles cibles en oncologie [VINCO]
dc.contributor.authorBONNET, Jacques
hal.structure.identifierPlateforme génomique fonctionnelle [PGFB]
hal.structure.identifierSociété IMAGENE
dc.contributor.authorCOLOTTE, Marthe
hal.structure.identifierSociété IMAGENE
dc.contributor.authorCOUDY, Delphine
hal.structure.identifierInstitut de Mathématiques de Bordeaux [IMB]
dc.contributor.authorCOUALLIER, Vincent
hal.structure.identifierInstitut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
dc.contributor.authorPORTIER, Josik
hal.structure.identifierInstitut des Sciences Moléculaires [ISM]
dc.contributor.authorMORIN, Bénédicte
hal.structure.identifierSociété IMAGENE
dc.contributor.authorTUFFET, Sophie
dc.date.issued2010
dc.identifier.issn0305-1048
dc.description.abstractEnThere is currently wide interest in room temperature storage of dehydrated DNA. However, there is insufficient knowledge about its chemical and structural stability. Here, we show that solid-state DNA degradation is greatly affected by atmospheric water and oxygen at room temperature. In these conditions DNA can even be lost by aggregation. These are major concerns since laboratory plastic ware is not airtight. Chain-breaking rates measured between 70 C and 140 C seemed to follow Arrhenius' law. Extrapolation to 25 C gave a degradation rate of about 1-40 cuts/105 nucleotides/ century. However, these figures are to be taken as very tentative since they depend on the validity of the extrapolation and the positive or negative effect of contaminants, buffers or additives. Regarding the secondary structure, denaturation experiments showed that DNA secondary structure could be preserved or fully restored upon rehydration, except possibly for small fragments. Indeed, below about 500 bp, DNA fragments underwent a very slow evolution (almost suppressed in the presence of trehalose) which could end in an irreversible denaturation. Thus, this work validates using room temperature for storage of DNA if completely protected from water and oxygen.
dc.language.isoen
dc.publisherOxford University Press
dc.subject.enDNA degradation
dc.subject.enChain-breaking rates
dc.subject.enAncient DNA
dc.subject.enAccelerated degradation
dc.subject.enArrhenius' law
dc.title.enChain and conformation stability of solid-state DNA: implications for room temperature storage
dc.typeArticle de revue
dc.identifier.doi10.1093/nar/gkp1060
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie structurale [q-bio.BM]
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
bordeaux.journalNucleic Acids Research
bordeaux.page1531-1546
bordeaux.volume38
bordeaux.issue5
bordeaux.peerReviewedoui
hal.identifierhal-00968097
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00968097v1
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