STEENTJES, Maikel B. F.; HERRERA VALDERRAMA, Andrea L.; FOUILLEN, Laetitia; BAHAMMOU, Delphine; LEISEN, Thomas; ALBERT, Isabell; NURNBERGER, Thorsten; HAHN, Matthias; MONGRAND, Sebastien; SCHOLTEN, Olga E.; VAN KAN, Jan A. L.

doi:10.1111/nph.18146

Laboratoire de biogenèse membranaire [LBM]

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Ce document a été publié dans

New Phytologist. 2022-07-01, vol. 235, n° 2, p. 690-700

Résumé en anglais

Necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are found throughout several plant-associated microbial taxa and are typically considered to possess cytolytic activity exclusively on dicot plant species. However, cytolytic NLPs are also produced by pathogens of monocot plants such as the onion (Allium cepa) pathogen Botrytis squamosa. We determined the cytotoxic activity of B. squamosa BsNep1, as well as other previously characterized NLPs, on various monocot plant species and assessed the plant plasma membrane components required for NLP sensitivity. Leaf infiltration of NLPs showed that onion cultivars are differentially sensitive to NLPs, and analysis of their sphingolipid content revealed that the GIPC series A : series B ratio did not correlate to NLP sensitivity. A tri-hybrid population derived from a cross between onion and two wild relatives showed variation in NLP sensitivity within the population. We identified a quantitative trait locus (QTL) for NLP insensitivity that colocalized with a previously identified QTL for B. squamosa resistance and the segregating trait of NLP insensitivity correlated with the sphingolipid content. Our results demonstrate the cytotoxic activity of NLPs on several monocot plant species and legitimize their presence in monocot-specific plant pathogens.< Réduire

Mots clés en anglais

Peptides

Plant Leaves

Plants

Proteins

Sphingolipids

URI

https://oskar-bordeaux.fr/handle/20.500.12278/140414

DOI

http://dx.doi.org/10.1111/nph.18146

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200

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Cytotoxic activity of Nep1-like proteins on monocots.