EZAN, Jerome; LASVAUX, Lea; GEZER, Aysegul; NOVAKOVIC, Ana; MAY-SIMERA, Helen; BELOTTI, Edwige; LHOUMEAU, Anne-Catherine; BIRNBAUMER, Lutz; BEER-HAMMER, Sandra; BORG, Jean-Paul; LE BIVIC, Andre; NÜRNBERG, Bernd; SANS, Nathalie; MONTCOUQUIOL, Mireille

doi:10.1038/ncb2819

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EZAN, Jerome

Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

LASVAUX, Lea
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

GEZER, Aysegul
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

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Article de revue

Ce document a été publié dans

Nature Cell Biology. 2013-09, vol. 15, n° 9, p. 1107-1115

Résumé en anglais

In ciliated mammalian cells, the precise migration of the primary cilium at the apical surface of the cells, also referred to as translational polarity, defines planar cell polarity (PCP) in very early stages. Recent research has revealed a co-dependence between planar polarization of some cell types and cilium positioning at the surface of cells. This important role of the primary cilium in mammalian cells is in contrast with its absence from Drosophila melanogaster PCP establishment. Here, we show that deletion of GTP-binding protein alpha-i subunit 3 (Gαi3) and mammalian Partner of inscuteable (mPins) disrupts the migration of the kinocilium at the surface of cochlear hair cells and affects hair bundle orientation and shape. Inhibition of G-protein function in vitro leads to kinocilium migration defects, PCP phenotype and abnormal hair bundle morphology. We show that Gαi3/mPins are expressed in an apical and distal asymmetrical domain, which is opposite and complementary to an aPKC/Par-3/Par-6b expression domain, and non-overlapping with the core PCP protein Vangl2. Thus G-protein-dependent signalling controls the migration of the cilium cell autonomously, whereas core PCP signalling controls long-range tissue PCP.< Réduire

URI

https://oskar-bordeaux.fr/handle/20.500.12278/140358

DOI

http://dx.doi.org/10.1038/ncb2819

Project ANR

Caractérisation moléculaire et fonctionnelle de vangl2 et de ses partenaires chez le mammifère

Unités de recherche

Neurocentre Magendie - U1215