SIMON, Marina; SAEZ, Gladys; MUGGIOLU, Giovanna; LAVENAS, Magali; LE TREQUESSER, Quentin; MICHELET, Claire; DEVÈS, Guillaume; BARBERET, Philippe; CHEVET, Eric; DUPUY, Denis; DELVILLE, Marie-Hélène; SEZNEC, Hervé

doi:10.1080/17435390.2017.1278803

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SIMON, Marina
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

SAEZ, Gladys
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

MUGGIOLU, Giovanna
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

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Article de revue

Ce document a été publié dans

Nanotoxicology. 2017-01-02, vol. 11, n° 1, p. 134-145

Taylor & Francis

Résumé en anglais

Although titanium dioxide nanoparticles (TiO2 NPs) have been extensively studied, their possible impact on health due to their specific properties supported by their size and geometry, remains to be fully characterized to support risk assessment. To further document NPs biological effects, we investigated the impact of TiO2 NPs morphology on biological outcomes. To this end, TiO2 NPs were synthesized as nanoneedles (NNs), titanate scrolled nanosheets (TNs), gel-sol-based isotropic nanoparticles (INPs) and tested for perturbation of cellular homeostasis (cellular ion content, cell proliferation, stress pathways) in three cell types and compared to the P25. We showed that TiO2 NPs were internalized at various degrees and their toxicity depended on both titanium content and NPs shape, which impacted on intracellular calcium homeostasis thereby leading to endoplasmic reticulum stress. Finally, we showed that a minimal intracellular content of TiO2 NPs was mandatory to induce toxicity enlightening once more the crucial notion of internalized dose threshold beside the well-recognized dose of exposure.< Réduire

Mots clés en anglais

ER stress

morphology-dependant toxicology

Titanium dioxide nanoparticles

calcium homeostasis

in situ quantification

DOI

http://dx.doi.org/10.1080/17435390.2017.1278803

Projet Européen

Support of Public and Industrial Research using Ion Beam Technology

Project ANR

Mécanismes d'internalisation et de toxicité des nanoparticules d'oxyde de titane dans des organismes multicellulaires eucaryotes

Origine

Importé de hal

Unités de recherche

Centre d'Études Nucléaires de Bordeaux Gradignan (CENBG)