In situ quantification of diverse titanium dioxide nanoparticles unveils selective endoplasmic reticulum stress-dependent toxicity
LE TREQUESSER, Quentin
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
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Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
Institut de Chimie de la Matière Condensée de Bordeaux [ICMCB]
Language
en
Article de revue
This item was published in
Nanotoxicology. 2017-01-02, vol. 11, n° 1, p. 134-145
Taylor & Francis
English Abstract
Although titanium dioxide nanoparticles (TiO2 NPs) have been extensively studied, their possible impact on health due to their specific properties supported by their size and geometry, remains to be fully characterized to ...Read more >
Although titanium dioxide nanoparticles (TiO2 NPs) have been extensively studied, their possible impact on health due to their specific properties supported by their size and geometry, remains to be fully characterized to support risk assessment. To further document NPs biological effects, we investigated the impact of TiO2 NPs morphology on biological outcomes. To this end, TiO2 NPs were synthesized as nanoneedles (NNs), titanate scrolled nanosheets (TNs), gel-sol-based isotropic nanoparticles (INPs) and tested for perturbation of cellular homeostasis (cellular ion content, cell proliferation, stress pathways) in three cell types and compared to the P25. We showed that TiO2 NPs were internalized at various degrees and their toxicity depended on both titanium content and NPs shape, which impacted on intracellular calcium homeostasis thereby leading to endoplasmic reticulum stress. Finally, we showed that a minimal intracellular content of TiO2 NPs was mandatory to induce toxicity enlightening once more the crucial notion of internalized dose threshold beside the well-recognized dose of exposure.Read less <
English Keywords
ER stress
morphology-dependant toxicology
Titanium dioxide nanoparticles
calcium homeostasis
in situ quantification
European Project
Support of Public and Industrial Research using Ion Beam Technology
ANR Project
Mécanismes d'internalisation et de toxicité des nanoparticules d'oxyde de titane dans des organismes multicellulaires eucaryotes
Origin
Hal imported