Single $\alpha$-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | MUGGIOLU, Giovanna | |
hal.structure.identifier | Laboratoire d'Intégration des Systèmes et des Technologies [LIST (CEA)] | |
dc.contributor.author | POMORSKI, Michal | |
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | CLAVERIE, Gérard | |
hal.structure.identifier | Laboratoire d'Intégration des Systèmes et des Technologies [LIST (CEA)] | |
dc.contributor.author | BERTHET, Guillaume | |
hal.structure.identifier | Laboratoire d'Intégration des Systèmes et des Technologies [LIST (CEA)] | |
dc.contributor.author | MER-CALFATI, Christine | |
hal.structure.identifier | Laboratoire d'Intégration des Systèmes et des Technologies [LIST (CEA)] | |
dc.contributor.author | SAADA, Samuel | |
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | DEVÈS, Guillaume | |
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | SIMON, Marina | |
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | SEZNEC, Herve | |
hal.structure.identifier | Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG] | |
dc.contributor.author | BARBERET, Philippe | |
dc.date.accessioned | 2022-04-19T12:59:32Z | |
dc.date.available | 2022-04-19T12:59:32Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/137274 | |
dc.description.abstractEn | As well as being a significant source of environmental radiation exposure, α-particles are increasinglyconsidered for use in targeted radiation therapy. A better understanding of α-particle induced damageat the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focusedα-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limitedrange, α-particles detection, delivery, and follow-up observations of radiation-induced damage remaindifficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane thatallows reliable single α-particles detection and single cell irradiation with negligible beam scattering.The radiation-induced responses of single 3MeV α-particles delivered with focused microbeam arevisualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8protein at DNA damaged sites. | |
dc.language.iso | en | |
dc.publisher | Nature Publishing Group | |
dc.title.en | Single $\alpha$-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1038/srep41764 | |
dc.subject.hal | Physique [physics] | |
bordeaux.journal | Scientific Reports | |
bordeaux.page | 41764 | |
bordeaux.volume | 7 | |
bordeaux.hal.laboratories | Centre d'Études Nucléaires de Bordeaux Gradignan (CENBG) | * |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-01763042 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-01763042v1 | |
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