DOMART, Florelle; CLOETENS, Peter; ROUDEAU, Stéphane; CARMONA, Asuncion; VERDIER, Emeline; CHOQUET, Daniel; ORTEGA, Richard

doi:10.7554/eLife.62334

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DOMART, Florelle
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

CLOETENS, Peter
European Synchrotron Radiation Facility [ESRF]

ROUDEAU, Stéphane
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

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eLife. 2020, vol. 9, p. e62334

eLife Sciences Publication

Résumé en anglais

Zinc and copper are involved in neuronal differentiation and synaptic plasticity but the molecular mechanisms behind these processes are still elusive due in part to the difficulty of imaging trace metals together with proteins at the synaptic level. We correlate stimulated emission depletion (STED) microscopy of proteins and synchrotron X-ray fluorescence (SXRF) imaging of trace metals, both performed with 40 nm spatial resolution, on primary rat hippocampal neurons. We achieve a detection limit for zinc of 14 zeptogram (10 -21 g) per pixel. We reveal the co-localization at the nanoscale of zinc and tubulin in dendrites with a molecular ratio of about one zinc atom per tubulin-αβ dimer. We observe the co-segregation of copper and F-actin within the nano-architecture of dendritic protrusions. In addition, zinc chelation causes a decrease in the expression of cytoskeleton proteins in dendrites and spines. Overall, these results indicate new functions for zinc and copper in the modulation of the cytoskeleton morphology in dendrites, a mechanism associated to neuronal plasticity and memory formation.< Réduire

Mots clés en anglais

zinc

copper

dendrite

spine

tubulin

actin

zinc

STED

synchrotron XRF

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Correlating STED and synchrotron XRF nano-imaging unveils the cosegregation of metals and cytoskeleton proteins in dendrites

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