DOMART, Florelle; CLOETENS, Peter; ROUDEAU, Stephane; CARMONA, Asuncion; VERDIER, Emeline; CHOQUET, Daniel; ORTEGA, Richard

doi:10.7554/elife.62334

Metadatos

Mostrar el registro completo del ítem

Licencia de uso del documento

DOMART, Florelle
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
Université de Bordeaux [UB]

CLOETENS, Peter
European Synchrotron Radiation Facility [ESRF]

ROUDEAU, Stephane
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

Idioma

Article de revue

Este ítem está publicado en

eLife. 2020, vol. 9, p. e62334

eLife Sciences Publication

Resumen en inglés

Zinc and copper are involved in neuronal differentiation and synaptic plasticity but the molecular mechanisms behind these processes are still elusive due in part to the difficulty of imaging trace metals together with proteins at the synaptic level. We correlate stimulated-emission-depletion microscopy of proteins and synchrotron X-ray fluorescence imaging of trace metals, both performed with 40 nm spatial resolution, on primary rat hippocampal neurons. We reveal the co-localization at the nanoscale of zinc and tubulin in dendrites with a molecular ratio of about one zinc atom per tubulin-αβ dimer. We observe the co-segregation of copper and F-actin within the nano-architecture of dendritic protrusions. In addition, zinc chelation causes a decrease in the expression of cytoskeleton proteins in dendrites and spines. Overall, these results indicate new functions for zinc and copper in the modulation of the cytoskeleton morphology in dendrites, a mechanism associated to neuronal plasticity and memory formation.< Leer menos

Metadatos

Compartir este ítem

Licencia de uso del documento

Correlating STED and synchrotron XRF nano-imaging unveils cosegregation of metals and cytoskeleton proteins in dendrites

Idioma

Este ítem está publicado en

Resumen en inglés

URI

DOI

Orígen

Centros de investigación