FU, Tingting; KNITTELFELDER, Oskar; GEFFARD, Olivier; CLEMENT, Yohann; TESTET, Eric; ELIE, Nicolas; TOUBOUL, David; ABBACI, Khedidja; SHEVCHENKO, Andrei; LEMOINE, Jerome; CHAUMOT, Arnaud; SALVADOR, Arnaud; DEGLI ESPOSTI, Davide; AYCIRIEX, Sophie

doi:10.1016/j.isci.2021.102115

Langue

Article de revue

Ce document a été publié dans

iScience. 2021, vol. 24, n° 2

Résumé en anglais

Sentinel species are playing an indispensable role in monitoring environmental pollution in aquatic ecosystems. Many pollutants found in water prove to be endocrine disrupting chemicals that could cause disruptions in lipid homeostasis in aquatic species. A comprehensive profiling of the lipidome of these species is thus an essential step toward understanding the mechanism of toxicity induced by pollutants. Both the composition and spatial distribution of lipids in freshwater crustacean Gammarus fossarum were extensively examined herein. The baseline lipidome of gammarids of different sex and reproductive stages was established by high throughput shotgun lipidomics. Spatial lipid mapping by high resolution mass spectrometry imaging led to the discovery of sulfate-based lipids in hepato-pancreas and their accumulation in mature oocytes. A diverse and dynamic lipid composition in G. fossarum was uncovered, which deepens our understanding of the biochemical changes during development and which could serve as a reference for future ecotoxicological studies.< Réduire

Mots clés en anglais

NM ultraviolet photodissociation

Ovarian maturation

Structural-characterization

Crude extracts

Accumulation

Amphipoda

Crustacea

Crab

Classification

URI

https://oskar-bordeaux.fr/handle/20.500.12278/136450

DOI

http://dx.doi.org/10.1016/j.isci.2021.102115

Project ANR

Approches Protéomique et Lipidomique pour la compréhension des mécanismes moléculaires de toxicité en lien avec l'altération du métabolisme lipidique chez l'espèce sentinelle Gammarus fossarum durant le cycle de reproduction - ANR-18-CE34-0008

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200

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Shotgun lipidomics and mass spectrometry imaging unveil diversity and dynamics in Gammarus fossarum lipid composition