SCHATZ, Christophe; DELAS, Tim

doi:10.1007/978-1-0716-1298-9_17

Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

DELAS, Tim

Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Langue

Chapitre d'ouvrage

Ce document a été publié dans

Design and Delivery of SiRNA Therapeutics, Methods in Molecular Biology book series. 2021-04-30, vol. 2282, p. 297-327

Springer US

Résumé en anglais

The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the formation of colloidal particles capable of delivering siRNA in vitro or in vivo. Among the polycations used for siRNA delivery, chitosan occupies a special place due to its unique physicochemical and biological properties. In this chapter we describe the fundamental and practical aspects of the formation of colloidal complexes between chitosan and siRNA. The basis of the electrostatic complexation between oppositely charged polyelectrolytes is first introduced with a focus on the specific conditions to obtain stable colloid complex particles. Subsequent, the properties that make chitosan so special are described. In a third part, the main parameters influencing the colloidal properties and stability of siRNA/chitosan complexes are reviewed with emphasis on some practical aspects to consider in the preparation of complexes.< Réduire

Mots clés en anglais

Chitosan

siRNA

Electrostatic interaction

Polyelectrolyte complexation

Complex particles

Biocolloids

URI

https://oskar-bordeaux.fr/handle/20.500.12278/124337

DOI

http://dx.doi.org/10.1007/978-1-0716-1298-9_17

Unités de recherche

Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche