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QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine

dc.rights.licenseopenen_US
dc.contributor.authorBARTLE, L.
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorPELTIER, Emilien
dc.contributor.authorSUNDSTROM, J.F.
dc.contributor.authorSUMBY, K.
dc.contributor.authorMITCHELL, J.G.
dc.contributor.authorJIRANEK, V.
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARULLO, Philippe
dc.date.accessioned2021-12-16T10:13:27Z
dc.date.available2021-12-16T10:13:27Z
dc.date.issued2021
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/124182
dc.description.abstractEnAbstract: The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions. Key points: - For the first time, QTL analysis has been used to study yeast-bacteria interactions. - A QTL encompassing a translocation, XV-t-XVI, was linked to MLF outcomes. - S. cerevisiae SSU1 haploinsufficiency positively impacted MLF by O. oeni.
dc.language.isoENen_US
dc.subject.enYeast-bacteria interactions
dc.subject.enMalolactic fermentation
dc.subject.enQTL
dc.subject.enWine
dc.subject.enSSU1
dc.title.enQTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s00253-021-11376-xen_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalApplied Microbiology and Biotechnologyen_US
bordeaux.page5053-5066en_US
bordeaux.volume105en_US
bordeaux.hal.laboratoriesUnité de Recherche Oenologie - EA 4577en_US
bordeaux.issue12en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDWine Australiaen_US
bordeaux.identifier.funderIDConseil Régional Aquitaineen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applied%20Microbiology%20and%20Biotechnology&rft.date=2021&rft.volume=105&rft.issue=12&rft.spage=5053-5066&rft.epage=5053-5066&rft.au=BARTLE,%20L.&PELTIER,%20Emilien&SUNDSTROM,%20J.F.&SUMBY,%20K.&MITCHELL,%20J.G.&rft.genre=article


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