Glycerol suppresses glucose consumption in trypanosomes through metabolic contest.
dc.rights.license | open | en_US |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | ALLMANN, Stefan | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | WARGNIES, Marion | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | PLAZOLLES, Nicolas | |
dc.contributor.author | CAHOREAU, Edern | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | BIRAN, Marc | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
dc.contributor.author | MORAND, Pauline | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | PINEDA, Erika | |
dc.contributor.author | KULYK, Hanna | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | ASENCIO, Corinne | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | VILLAFRAZ, Oriana | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | RIVIÈRE, Loïc | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | TETAUD, Emmanuel | |
dc.contributor.author | ROTUREAU, Brice | |
hal.structure.identifier | Institut de biochimie et génétique cellulaires [IBGC] | |
dc.contributor.author | MOURIER, Arnaud | |
dc.contributor.author | PORTAIS, Jean-Charles | |
hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
dc.contributor.author | BRINGAUD, Frédéric | |
dc.date.accessioned | 2021-10-06T06:35:24Z | |
dc.date.available | 2021-10-06T06:35:24Z | |
dc.date.issued | 2021-08-01 | |
dc.identifier.issn | 1545-7885 | en_US |
dc.identifier.other | https://figshare.com/articles/journal_contribution/Growth_curves_of_the_parental_WT_and_the_tetracycline-induced_sup_i_RNAi_i_sup_GK_i_cell_lines_maintained_in_the_presence_of_10_mM_glucose_and_glycerol_Glc_Glyc_10_mM_glucose_Glc_Glyc_10_mM_glycerol_Glc_Glyc_or_none_of_them_Glc_Glyc_/15166659 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/112555 | |
dc.description.abstractEn | Microorganisms must make the right choice for nutrient consumption to adapt to their changing environment. As a consequence, bacteria and yeasts have developed regulatory mechanisms involving nutrient sensing and signaling, known as "catabolite repression," allowing redirection of cell metabolism to maximize the consumption of an energy-efficient carbon source. Here, we report a new mechanism named "metabolic contest" for regulating the use of carbon sources without nutrient sensing and signaling. Trypanosoma brucei is a unicellular eukaryote transmitted by tsetse flies and causing human African trypanosomiasis, or sleeping sickness. We showed that, in contrast to most microorganisms, the insect stages of this parasite developed a preference for glycerol over glucose, with glucose consumption beginning after the depletion of glycerol present in the medium. This "metabolic contest" depends on the combination of 3 conditions: (i) the sequestration of both metabolic pathways in the same subcellular compartment, here in the peroxisomal-related organelles named glycosomes; (ii) the competition for the same substrate, here ATP, with the first enzymatic step of the glycerol and glucose metabolic pathways both being ATP-dependent (glycerol kinase and hexokinase, respectively); and (iii) an unbalanced activity between the competing enzymes, here the glycerol kinase activity being approximately 80-fold higher than the hexokinase activity. As predicted by our model, an approximately 50-fold down-regulation of the GK expression abolished the preference for glycerol over glucose, with glucose and glycerol being metabolized concomitantly. In theory, a metabolic contest could be found in any organism provided that the 3 conditions listed above are met. | |
dc.language.iso | EN | en_US |
dc.title.en | Glycerol suppresses glucose consumption in trypanosomes through metabolic contest. | |
dc.title.alternative | PLoS Biol | en_US |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1371/journal.pbio.3001359 | en_US |
dc.subject.hal | Sciences du Vivant [q-bio]/Ingénierie biomédicale | |
dc.identifier.pubmed | 34388147 | en_US |
bordeaux.journal | Plos Biology | en_US |
bordeaux.page | e3001359 | en_US |
bordeaux.volume | 19 | en_US |
bordeaux.hal.laboratories | Centre de Résonance Magnétique des Systèmes Biologiques (CRMSB) - UMR 5536 | en_US |
bordeaux.issue | 8 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | CNRS | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
bordeaux.import.source | pubmed | |
hal.export | false | |
workflow.import.source | pubmed | |
dc.rights.cc | Pas de Licence CC | en_US |
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