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Characterization of Tomato-associated Rhizobacteria Recovered from Various Tomato-growing Sites in Tunisia

dc.rights.licenseopenen_US
dc.contributor.authorABDELJALIL, Nada Ouhaibi-Ben
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorVALLANCE, Jessica
ORCID: 0000-0002-5581-4453
IDREF: 143291556
dc.contributor.authorGERBORE, Jonathan
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorBRUEZ, Emilie
IDREF: 16920491X
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARTINS, Guilherme
ORCID: 0000-0002-4049-3482
IDREF: 164009957
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorREY, Patrice
dc.contributor.authorDAAMI-REMADI, Mejda
dc.date.accessioned2021-09-10T13:32:11Z
dc.date.available2021-09-10T13:32:11Z
dc.date.issued2016-01-01
dc.identifier.issn2157-7471en_US
dc.identifier.urioai:crossref.org:10.4172/2157-7471.1000351
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112202
dc.description.abstractEnIn the present study, a total of 200 rhizobacterial isolates were obtained from rhizosphere of healthy tomato plants grown in fields with a history of severe soilborne diseases and mainly crown and root rots. Screened their capacity to suppress in vitro growth of Sclerotinia sclerotiorum and Rhizoctonia solani, 69 and 57 isolates out of the 200 tested were shown able to inhibit significantly the mycelial growth of target pathogens by11-62% relative to control. The 25 most effective isolates, leading to suppression of both fungi by more than 45% over control, were selected and subjected to morphological, biochemical, molecular, and metabolic characterizations. This collection of tomato-associated rhizobacteria exhibited a great morphological and biochemical diversity. Sequencing of 16S rRNA and rpoB genes led to the identification of four genera namely Bacillus, Chryseobacterium, Enterobacter, and Klebsiella. The most frequent species were B. amyloliquefaciens, B. thuringiensis, B. megaterium, B. subtilis, E. cloacae, C. jejuense,and K. pneumoniae. Screening for their plant growth-promoting properties, 20 isolates were shown able to produce siderophore, 18 had solubilized phosphate, and 19 were capable to synthesize indole-3-acetic acid (IAA). PCR amplification of lipopeptide biosynthetic genes revealed the presence of genes encoding fengycin A and bacillomycin D biosynthesis in 18 and 16 isolates, respectively. Metabolic characterization performed using Biolog™ Ecoplates indicated that tomato-associated rhizobacteria displayed a large metabolic activity and they were able to use a wide range of carbon sources with the increase of the incubation duration. Based on their metabolic profiles, these rhizobacterial isolates were grouped into eight major clusters generated at the different sampling times (24, 48 and 120 h of incubation). Average well-color development (AWCD) values were found to be positively correlated with the Shannon diversity index.
dc.language.isoENen_US
dc.sourcecrossref
dc.subjectAntifungal activity
dc.subjectCharacterization
dc.subjectDiversity
dc.subjectIdentification
dc.subjectRhizobacteria
dc.subjectTomato
dc.subjectTunisia
dc.title.enCharacterization of Tomato-associated Rhizobacteria Recovered from Various Tomato-growing Sites in Tunisia
dc.title.alternativeJ Plant Pathol Microbiolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.4172/2157-7471.1000351en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalJournal of Plant Pathology and Microbiologyen_US
bordeaux.volume07en_US
bordeaux.hal.laboratoriesUnité de Recherche Oenologie - EA 4577en_US
bordeaux.issue05en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Plant%20Pathology%20and%20Microbiology&rft.date=2016-01-01&rft.volume=07&rft.issue=05&rft.eissn=2157-7471&rft.issn=2157-7471&rft.au=ABDELJALIL,%20Nada%20Ouhaibi-Ben&VALLANCE,%20Jessica&GERBORE,%20Jonathan&BRUEZ,%20Emilie&MARTINS,%20Guilherme&rft.genre=article


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