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Seasonal variations in the metabolome and bioactivity profile of Fucus vesiculosus extracted by an optimised, pressurised liquid extraction protocol

dc.rights.licenseopenen_US
dc.contributor.authorHEAVISIDES, Edwin
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorROUGER, Caroline
IDREF: 158011724
dc.contributor.authorREICHEL, Anna F.
dc.contributor.authorULRICH, Cornelia
dc.contributor.authorWENZEL-STORJOHANN, Arlette
dc.contributor.authorSEBENS, Susanne
dc.contributor.authorTASDEMIR, Deniz
dc.date.accessioned2020-09-02T08:20:35Z
dc.date.available2020-09-02T08:20:35Z
dc.date.issued2018
dc.identifier.issn1660-3397en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10757
dc.description.abstractEnThe metabolism of seaweeds depends on environmental parameters, the availability of nutrients, and biotic/abiotic stresses; therefore, their chemical composition fluctuates throughout the year. This study investigated seasonal variations in the metabolome of the Baltic Sea brown alga Fucus vesiculosus and its potential relation to the bioactivity profile. By using a definitive screening design (DSD) combined with pressurised liquid extraction (PLE), an optimised protocol was developed to extract algal biomass monthly for a full calendar year. An untargeted metabolomics approach using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MSn)-based molecular networking and manual dereplication was employed. The extracts were simultaneously screened for their in vitro antimicrobial, anticancer/apoptotic, and free radical scavenging activities. 44 compounds were putatively dereplicated in the metabolome. Many compounds were found to vary with the sampling month; phlorotannin total ion count (TIC) was highest in summer, whilst chlorophylls, lipids, and carotenoids peaked in winter and spring. The greatest radical scavenging and apoptotic activities against pancreas cancer cells observed in the summer months were attributed to high phlorotannin TIC. Methicillin-resistant Staphylococcus aureus (MRSA) inhibitory activity was produced year-round without a clear seasonal trend. This is the first study applying DSD-based optimised PLE extraction combined with a metabolome analysis of F. vesiculosus for the identification of seasonal variations in both metabolome and bioactivity.
dc.language.isoENen_US
dc.subject.enFucus Vesiculosus
dc.subject.enBioactivity
dc.subject.enBladder Wrack
dc.subject.enDefinitive Screening Design
dc.subject.enMacroalga
dc.subject.enPressurised Liquid Extraction
dc.subject.enSeasonal Variation
dc.subject.enUntargeted Metabolomics
dc.title.enSeasonal variations in the metabolome and bioactivity profile of Fucus vesiculosus extracted by an optimised, pressurised liquid extraction protocol
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/md16120503en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed30551573en_US
bordeaux.journalMarine Drugsen_US
bordeaux.page1-28en_US
bordeaux.volume16en_US
bordeaux.hal.laboratoriesOenologie - EA 4577en_US
bordeaux.issue12en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine%20Drugs&rft.date=2018&rft.volume=16&rft.issue=12&rft.spage=1-28&rft.epage=1-28&rft.eissn=1660-3397&rft.issn=1660-3397&rft.au=HEAVISIDES,%20Edwin&ROUGER,%20Caroline&REICHEL,%20Anna%20F.&ULRICH,%20Cornelia&WENZEL-STORJOHANN,%20Arlette&rft.genre=article


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