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Melatonin, protocatechuic acid and hydroxytyrosol effects on vitagenes system against alpha-synuclein toxicity

dc.rights.licenseopenen_US
dc.contributor.authorGALLARDO-FERNANDEZ, Marta
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorHORNEDO ORTEGA, Ruth
dc.contributor.authorCEREZO, Ana B.
dc.contributor.authorTRONCOSO, Ana M.
dc.contributor.authorGARCIA-PARRILLA, M. Carmen
dc.date.accessioned2020-07-07T09:38:17Z
dc.date.available2020-07-07T09:38:17Z
dc.date.issued2019
dc.identifier.issn0278-6915en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10172
dc.description.abstractEnPreventing the abnormal assembly of α-synuclein (α-Syn) and the correct modulation of vitagenes system exercise strong neuroprotective effects. It has been reported that melatonin (MEL), protocatechuic acid (PCA) and hydroxytyrosol (HT) reduce α-Syn toxicity. Their effect on the vitagenes system of PC12 cells have not been explored yet. These bioactive can cross the blood brain barrier (BBB). Therefore, this work aims to evaluate the inhibitory and destabilising capacities of MEL, PCA, HT, and their combinations on α-Syn kinetics and effects on vitagenes system (sirtuin-1 (SIRT-1), sirtuin-2 (SIRT-2), heme oxygenase (HO-1) and heat shock protein 70 (Hsp-70)). In vitro techniques (Thioflavin T (ThT), Transmission Electronic Microscopy (TEM), electrophoresis, MTT assay and qPCR) were used. Compounds, both individually and simultaneously were able to decrease the toxicity induced by α-Syn. Concurrently, occurrence of PCA (100 μM) +HT (100 μM) showed the highest inhibitory effect against α-Syn fibril formation and destabilisation of α-Syn fibrils (88 and 62%, respectively). Moreover, these compounds increased the expression of SIRT-2, HO-1 and Hsp70, contributing to a neuroprotective effect. In addition, the most important result is the increase on the expression of SIRT-2 caused by the combination of MEL + HT + PCA in the absence of α-Syn fibrils.
dc.language.isoENen_US
dc.subject.enHydroxytyrosol
dc.subject.enMelatonin
dc.subject.enParkinson's Disease
dc.subject.enProtocatechuic Acid
dc.subject.enVitagenes System
dc.subject.enΑ-Synuclein
dc.title.enMelatonin, protocatechuic acid and hydroxytyrosol effects on vitagenes system against alpha-synuclein toxicity
dc.title.alternativeFood chem. toxicol.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.fct.2019.110817en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed31521636en_US
bordeaux.journalFood and Chemical Toxicologyen_US
bordeaux.page1-11en_US
bordeaux.volume134en_US
bordeaux.hal.laboratoriesOenologie - EA 4577en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Food%20and%20Chemical%20Toxicology&rft.date=2019&rft.volume=134&rft.spage=1-11&rft.epage=1-11&rft.eissn=0278-6915&rft.issn=0278-6915&rft.au=GALLARDO-FERNANDEZ,%20Marta&HORNEDO%20ORTEGA,%20Ruth&CEREZO,%20Ana%20B.&TRONCOSO,%20Ana%20M.&GARCIA-PARRILLA,%20M.%20Carmen&rft.genre=article


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