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Photochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station

hal.structure.identifierUniversité Montpellier 2 - Sciences et Techniques [UM2]
dc.contributor.authorCOUSSOT, Gaëlle
hal.structure.identifierLaboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
dc.contributor.authorLE POSTOLLEC, Aurélie
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorINCERTI, Sébastien
dc.contributor.authorBAQUÉ, Mickaël
dc.contributor.authorFAYE, Clément
hal.structure.identifierUniversité de Brest [UBO]
dc.contributor.authorVANDENABEELE-TRAMBOUZE, Odile
hal.structure.identifierLaboratoire Interuniversitaire des Systèmes Atmosphériques [LISA (UMR_7583)]
dc.contributor.authorCOTTIN, Hervé
hal.structure.identifierUniversité Grenoble Alpes [2016-2019] [UGA [2016-2019]]
dc.contributor.authorRAVELET, Corinne
hal.structure.identifierUniversité Grenoble Alpes [2016-2019] [UGA [2016-2019]]
dc.contributor.authorPEYRIN, Eric
hal.structure.identifierUniversité Grenoble Alpes [2016-2019] [UGA [2016-2019]]
dc.contributor.authorFIORE, Emmanuelle
dc.contributor.authorVIGIER, Flavie
dc.contributor.authorCARON, Jérôme
hal.structure.identifierCentre National d'Études Spatiales [Toulouse] [CNES]
dc.contributor.authorCHAPUT, Didier
dc.contributor.authorPRZYBYLA, Bartos
dc.contributor.authorBERGER, Thomas
hal.structure.identifierLaboratoire d'Astrophysique de Bordeaux [Pessac] [LAB]
dc.contributor.authorDOBRIJEVIC, Michel
dc.date.issued2019
dc.description.abstractEnSome microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to Mars. We found that the recognition ability of fluorescently labeled aptamers was not significantly affected during short-term exposure experiments taking into account only one type of radiation at a time. However, we demonstrated that the same fluorescent dye was significantly affected by temperature variations (−21°C to +58°C) and storage throughout the entirety of the ISS experiment (60% of signal loss). This induced a large variability of aptamer signal in our analysis. However, we found that >50% of aptamers were still functional after the whole EXPOSE-R2 mission. We conclude that aptamer-based instruments are well suited for in situ analysis on planetary bodies, but the detection step requires additional investigations.
dc.language.isoen
dc.title.enPhotochemistry on the Space Station—Aptamer Resistance to Space Conditions: Particles Exposure from Irradiation Facilities and Real Exposure Outside the International Space Station
dc.typeArticle de revue
dc.identifier.doi10.1089/ast.2018.1896
dc.subject.halPhysique [physics]
bordeaux.journalAstrobiology
bordeaux.page1063-1074
bordeaux.volume19
bordeaux.issue8
bordeaux.peerReviewedoui
hal.identifierhal-02272610
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02272610v1
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