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Chitosan-based hydrogels for developing a small-diameter vascular graft: in vitro and in vivo evaluation

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAUSSEL, A.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHEBAUD, N.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBÉRARD, B.
dc.contributor.authorBRIZZI, B.
hal.structure.identifierCIC Bordeaux
dc.contributor.authorDELMOND, D.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBAREILLE, B.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSIADOUS, R.
hal.structure.identifierBiologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorJAMES, J.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRIPOCHE, J.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorDURAND, D.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorMONTEMBAULT, A.
hal.structure.identifierCentre Technologique des Microstructures [CTµ]
dc.contributor.authorBURDIN, B
hal.structure.identifierLaboratoire de Recherche Vasculaire Translationnelle [LVTS (UMR_S_1148 / U1148)]
dc.contributor.authorLETOURNEUR, D.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, N.
hal.structure.identifierIngénierie des Matériaux Polymères [IMP]
dc.contributor.authorDAVID, L.
hal.structure.identifierService de médecine nucléaire [Bordeaux]
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorBORDENAVE, L.
dc.date.accessioned2021-06-10T07:05:41Z
dc.date.available2021-06-10T07:05:41Z
dc.date.issued2017-12-01
dc.identifier.issn1748-6041
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/79034
dc.description.abstractEnAims: Vascular grafts made of synthetic polymers perform poorly in small-diameter applications (cardiac and peripheral bypass). Chitosan is a biocompatible natural polymer that can provide a novel biological scaffold for tissue engineering development. The goal of this study was to demonstrate the biocompatibility of a novel chitosan preparation in vitro and in vivo, and to assess its potential as a scaffold for vascular applications.Methods and results: A series of experiments of increasing complexity, ranging from in vitro biocompatibility and hemocompatibility tests to in vivo studies in small and large animals (rats and sheep), was performed to provide a comprehensive analysis of chitosan hydrogels' biological properties. In vitro studies established that: (i) chitosan supported human endothelial progenitor cells adhesion, proliferation and resistance to physiological shear stress; (ii) chitosan did not activate platelets, the complement system, or the intrinsic coagulation pathway. In vivo results showed: (iii) no resorption of chitosan and no chronic inflammation at 60 days in a rat heterotopic implantation model (magnetic resonance imaging and histology); (iv) no flow obstruction (Doppler ultrasound) and no thrombus formation (histology and scanning electron microscopy) at 2 h after a carotid arteriotomy repair with chitosan patches in sheep. Finally, two chitosan tubes were implanted as carotid interposition grafts for 3 days in sheep showing that chitosan was strong enough to be sutured, to withstand arterial pressure, and no flow obstruction was observed through this short period.Conclusion: Chitosan-based hydrogels displayed promising in vitro biocompatibility and hemocompatibility properties as well as in vivo short-term performance.
dc.language.isoen
dc.publisherIOP Publishing
dc.title.enChitosan-based hydrogels for developing a small-diameter vascular graft: in vitro and in vivo evaluation
dc.typeArticle de revue
dc.identifier.doi10.1088/1748-605X/aa78d0
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
bordeaux.journalBiomedical Materials
bordeaux.page065003
bordeaux.volume12
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue6
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierhal-01659658
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01659658v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedical%20Materials&rft.date=2017-12-01&rft.volume=12&rft.issue=6&rft.spage=065003&rft.epage=065003&rft.eissn=1748-6041&rft.issn=1748-6041&rft.au=AUSSEL,%20A.&THEBAUD,%20N.&B%C3%89RARD,%20B.&BRIZZI,%20B.&DELMOND,%20D.&rft.genre=article


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