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Isolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties

hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorRÉMY, Murielle
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFERRARO, Francesca
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLE SALVER, Pierre
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
dc.contributor.authorGENOT, Elisabeth
hal.structure.identifierActions for OnCogenesis understanding and Target Identification in ONcology [ACTION]
dc.contributor.authorDJAVAHERI-MERGNY, Mojgan
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorTHÉBAUD, Noelie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOIZIAU, Claudine
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Hélène
ORCID: 0000-0002-3006-8773
IDREF: 03205453X
dc.date.accessioned2021-06-10T07:03:49Z
dc.date.available2021-06-10T07:03:49Z
dc.date.issued2019-11
dc.identifier.issn2073-4409
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78936
dc.description.abstractEnStem cells isolated from the apical papilla of wisdom teeth (SCAPs) are an attractive model for tissue repair due to their availability, high proliferation rate and potential to differentiate in vitro towards mesodermal and neurogenic lineages. Adult stem cells, such as SCAPs, develop in stem cell niches in which the oxygen concentration [O2] is low (3-8% compared with 21% of ambient air). In this work, we evaluate the impact of low [O2] on the physiology of SCAPs isolated and processed in parallel at 21% or 3% O2 without any hyperoxic shock in ambient air during the experiment performed at 3% O2. We demonstrate that SCAPs display a higher proliferation capacity at 3% O2 than in ambient air with elevated expression levels of two cell surface antigens: the alpha-6 integrin subunit (CD49f) and the embryonic stem cell marker (SSEA4). We show that the mesodermal differentiation potential of SCAPs is conserved at early passage in both [O2], but is partly lost at late passage and low [O2], conditions in which SCAPs proliferate efficiently without any sign of apoptosis. Unexpectedly, we show that autophagic flux is active in SCAPs irrespective of [O2] and that this process remains high in cells even after prolonged exposure to 3% O2.
dc.language.isoen
dc.publisherMDPI
dc.title.enIsolation and Culture of Human Stem Cells from Apical Papilla under Low Oxygen Concentration Highlight Original Properties
dc.typeArticle de revue
dc.identifier.doi10.3390/cells8121485
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalCells
bordeaux.page1485
bordeaux.volume8
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue12
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870962
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870962v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2019-11&rft.volume=8&rft.issue=12&rft.spage=1485&rft.epage=1485&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=R%C3%89MY,%20Murielle&FERRARO,%20Francesca&LE%20SALVER,%20Pierre&REY,%20Sylvie&GENOT,%20Elisabeth&rft.genre=article


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